A study of the initial adhesive force of cells on silk fibroin-based materials using micropipette aspiration  

作　　者：Xiaojie Lian Shichao Liu Liming Liu Rui Xu Miaomiao Du Song Wang Hesun Zhu Qiang Lu Quanyou Zhang Yali Wu Di Huang Yan Wei 

机构地区：[1]Department of Biomedical Engineering,Research Center for Nano-Biomaterials and Regenerative Medicine,College of Mechanics,Taiyuan University of Technology,Taiyuan 030024,China [2]Shanxi Key Laboratory of Material Strength and Structural Impact,Institute of Applied Mechanics and Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China [3]Research Center of Materials Science,School of Materials Science and Engineering,Beijing Institute of Technology,Beijing 100081,China [4]National Engineering Laboratory for Modern Silk,College of Textile and Clothing Engineering,Soochow University,Suzhou 215123,China

出　　处：《Regenerative Biomaterials》2018年第3期151-157,共7页再生生物材料（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(NSFC,no.51502192,11502158,31501212 and 51503140);Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(STIP,no.2016142);National Basic Research Program of China(973 project,2005CB623906);Natural Science Foundation for Young Scientists of Shanxi Province(no.2014021039-6 and 201601D021127);the Qualified Personnel Foundation of Taiyuan University of Technology(QPFT,no.tyut-rc201270a);the Youth Foundation of Taiyuan University of Technology(no.1205-04020102,2013Z020 and 2014TD066);the Technical Services Project of Taiyuan University of Technology(no.143230043-J).

摘　　要：With the development of biomaterials,more attention is paid to the adhesion characteristics between cells and materials.It is necessary to study the adhesive force with a suitable method.Silk fibroin(SF)is widely investigated in biomedical application due to its novel biocompatibility and mechanical properties.In this article,the micropipette aspiration method and measurement pattern of uniform cells in round shape(UCR)was used to study the initial adhesive force of three types of cells on pure silk fibroin films(SFFs).We also compared the adhesive forces of modified SFFs with that of pure SFFs.The results of adhesive force in the initial adhesive stage were in concordance with the results of MTT assay andmicroscope observation,which were confirmed by the above three cell lines and four kinds of SFFs.The results indicated UCR was an efficient and quantitative measurement pattern in initial adhesion stage.This article also provides a useful method in identifying initial cell-materials interactions.

关 键 词：silk-based materials cell initial adhesion micropipette uniform cells in round shape 

分 类 号：R73[医药卫生—肿瘤]