Human bone graft cytocompatibility with mesenchymal stromal cells is comparable after thermal sterilization and washing followed byγ-irradiation:an in vitro study  

作　　者：Dmitry Labutin Konstantin Vorobyov Svetlana Bozhkova Ekaterina Polyakova Tatyana Vodopyanova 

机构地区：[1]Division of Wound Infection Treatment and Prevention,Vreden Russian Research Institute of Traumatology and Orthopedics,St.Petersburg,Russian Federation

出　　处：《Regenerative Biomaterials》2018年第2期85-92,共8页再生生物材料（英文版）

基　　金：the Ministry of Health of the Russian Federation[grant no 115030510010].

摘　　要：Human bone allografts present a better alternative to autografts in terms of minimization of the harvesting procedure complications.Prior to the use in clinical applications,they require sterilization which aims to reduce bioburden.This often comes at the expense of their biological properties as carriers of cells.In this study,we evaluated the cytocompatibility of human bone allografts processed and sterilized by three different methods withmesenchymal stromal cells.Bone morphology,biological and biochemical properties of the extracted bone-conditionedmedium and viability of cells were assessed.We found that chemical sterilization had a strong negative effect on cell viability,whereas thermal sterilization and washing with subsequentγ-irradiation both resulted in a bone graft compatible with the progenitor cells.Moreover,washing of the bone prior to sterilization allowed solid removal of cell debris and other bone marrow components.Taken together,our findings demonstrate the importance of a proper choice of the bone graft processing method for the production of the biomaterial suitable for tissue engineering.

关 键 词：bone grafts bone sterilization mesenchymal stromal cells biocompatibility testing 

分 类 号：R73[医药卫生—肿瘤]