太平洋西部鳓鱼隐蔽种的界定及系统发育关系重建  

Cryptic Species and the Phylogenetic Relationships of the Ilisha elongata along the Northwestern Pacific Coast

作　　者：王倩[1] 李晨虹[1] Wang Qian;Li Chenhong(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai,201306)

机构地区：[1]上海海洋大学,水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《基因组学与应用生物学》2020年第12期5481-5487,共7页Genomics and Applied Biology

基　　金：上海市教育委员会科研创新项目(B2-5308-13-0455)资助。

摘　　要：鳓鱼(Ilisha elongata)属于鲱形目(Clupeiformes),是锯腹鳓科的鱼类之一,鳓鱼目前在世界上主要分布在太平洋西部和沙捞越爪哇海海域。本研究报道了来自丹东和烟台的鳓鱼完整的线粒体基因组序列。线粒体基因组长度为16809 bp,包括13个蛋白编码基因、22个tRNA、2个rRNA、12S rRNA、16S rRNA和控制区D-loop基因。本研究还对上述2个地点的6尾鳓鱼,利用完整的线粒体基因组以及非洲鳓作为外群重建了系统发育关系。结果显示,来自丹东和烟台的鳓鱼群体分成两个姊妹关系的单系群。用物种界定的方法(基于BEAST的GMYC模型)对丹东鳓鱼群体有较好的划分,研究显示丹东鳓鱼群体是太平洋西部的隐蔽种。由此推断,丹东鳓鱼群体隐蔽种的形成很可能是更新世冰期海平面下降产生了地理隔离以及后续长时间的基因交流障碍的结果。Ilisha elongate belongs to Clupeiformes,Pristigasteridae.It is mainly distributed in the western Pacific Ocean and the Sarawak Sea.Here we reported the complete mitogenome of Ilisha elongate,which were collected from Dandong and Yantai.The genome sequence is 16809 bp in length,which comprises 13 protein-coding genes,22 tRNAs,2 rRNAs,12 S rRNA,16 S rNA and a control region.The phylogenetic relationships of 6 Ilisha elongate from Dandong and Yantai were reconstructed based on the complete mitochondrial genome and the Ilisha africa as the outgroup.The results showed that the population of Dandong and Yantai were divided into two sisters monophyletic groups.We used BEAST Tree-based methods(GMYC)to infer the putative species group of Dandong.The phylogenetic analyses showed that the Dandong population is a cryptic species.Our findings suggested that species split between them was attributed to geographical isolation during lowing sea levels of ice ages and the barrier of gene flow of the ocean currents during interglacial period in the late Pliocene.

关 键 词：鳓鱼 隐蔽种 GMYC 线粒体基因组 系统发育关系 

分 类 号：S917.4[农业科学—水产科学]