Role of integrin in influencing differentiation of PC12 cell grown on PLLA-aligned nanofiber:a mRNA–microRNA–protein integrative study  

作　　者：Xiaoying Lu Fei Yang Yan Huang Yadong Yu 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,PR China [2]Co-innovation Center of Neuroregeneration,Nantong University,Nantong 226001,PR China

出　　处：《Regenerative Biomaterials》2017年第2期89-96,共8页再生生物材料（英文版）

基　　金：This work was supported by National Natural Science Foundation of China(31170910,31271012 and 31600816);the Natural Science Foundation of Jiangsu Province(BK20150599).

摘　　要：The aim of this study is to unveil the role of integrin in influencing the differentiation of PC12 cell grown on PLLA aligned nanofibers by integrative study of cDNA microarray,proteomics technology and microRNA sequencing technology.First,PLLA-aligned nanofibers were prepared by electrospinning,and the chemical composition and surface morphology of the nanofibers were examined.Then,morphology and neurite length of PC12 cells and GRGDS-treated PC12 cells on PLLA-aligned nanofibers(AF and GA group)were measured by high-content analysis system.Subsequently,protein expression profile was analyzed by iTRAQ proteomics technology and 250 differentially expressed proteins were screened in the GA group.Integrative analysis of mRNAmicroRNA-protein data showed that‘MAPK signaling pathway’was the key affected pathway after the function of integrin were interfered by GRGDS.Other seven pathways were also influenced,and the differentiation of PC12 cell grown on PLLA aligned nanofibers was finally inhibited.

关 键 词：INTEGRIN aligned nanofibers cell differentiation mRNA-microRNA-protein integrative analysis 

分 类 号：TB3[一般工业技术—材料科学与工程]