慢性肾脏病患者血清微小RNA-210表达水平及其与颈动脉粥样硬化的关系  

Serum microRNA-210 levels in patients with chronic kidney disease and its relation with carotid artery atherosclerosis

作　　者：布海霞[1] 徐可[1] 王树龙[2] Bu Haixia;Xu Ke;Wang Shulong(Department of Nephrology,Xinxiang Central Hospital,Xinxiang 453000,China;不详)

机构地区：[1]河南省新乡市中心医院肾脏内科,453000 [2]新乡医学院第一附属医院肾脏内科

出　　处：《临床内科杂志》2021年第3期174-177,共4页Journal of Clinical Internal Medicine

基　　金：河南省医学科技攻关计划普通项目(201702127)。

摘　　要：目的探讨慢性肾脏病(CKD)患者血清微小RNA(miR)-210表达水平及其与颈动脉粥样硬化的相关性。方法回顾性纳入130例CKD患者为CKD组,同期35例健康体检者为对照组。收集两组受试者的临床资料并比较,采用逆转录聚合酶链反应(RT-PCR)检测血清miR-210相对表达水平,采用彩色多普勒超声诊断仪测量颈动脉内膜中层厚度(IMT),根据颈动脉IMT将130例CKD患者再分为正常组(44例)、内膜增厚组(49例)和颈动脉粥样硬化斑块形成组(39例)并比较3组患者血清miR-210表达水平。采用Spearman相关分析探讨CKD患者血清miR-210相对表达水平与实验室检查结果的相关性,采用logistic回归分析探讨CKD患者颈动脉IMT的危险因素,采用受试者工作特征(ROC)曲线评估血清miR-210诊断CKD患者发生颈动脉粥样硬化的效能。结果与对照组相比,CKD组患者低密度脂蛋白胆固醇(LDL-C)、尿酸、血肌酐、颈动脉IMT、miR-210水平均显著升高,而Hb、白蛋白(Alb)、估算的肾小球滤过率(eGFR)水平均显著降低(P<0.05)。与正常组和内膜增厚组比较,颈动脉粥样硬化形成组患者血清miR-210相对表达水平均显著升高(P<0.001);与正常组相比,内膜增厚组患者血清miR-210相对表达水平显著升高(P<0.001)。CKD 1期、2期、3期、4期和5期患者血清miR-210相对表达水平间比较差异均有统计学意义(P<0.001)。Spearman相关分析结果显示,CKD患者血清miR-210相对表达水平与颈动脉IMT、LDL-C、血肌酐均呈显著正相关,而与eGFR呈现显著负相关(P<0.05)。Logistic回归分析结果显示,血肌酐、eGFR和miR-210均为CKD患者颈动脉IMT增厚的危险因素(P<0.001)。ROC曲线分析结果显示,血清miR-210诊断CKD患者发生颈动脉粥样硬化的ROC曲线下面积为0.902(95%CI 0.835~0.942,P<0.001),当截断值为2.31时,敏感度和特异度分别为93.24%和95.25%。结论CKD患者血清中miR-210相对表达水平显著升高,miR-210可能参与了CKD患者颈�Objective To investigate the expression of serum microRNA(miR)-210 in patients with chronic kidney disease(CKD)and its relation with carotid artery atherosclerosis.Methods A total of 130 patients with CKD(CKD group)and 35 healthy individuals(control group)were enrolled.The clinical data of the two groups were collected and compared.The serum miR-210 level and carotid intima-media thickness(IMT)were detected by RT-PCR and color Doppler ultrasonography,respectively.According to IMT of carotid artery,130 patients with CKD were divided into normal group(44 cases),intima thickening group(49 cases)and carotid atherosclerotic group(39 cases).The serum miR-210 level of the three groups were compared.Correlation between serum miR-210 level with biochemical indicators in patients with CKD were analyzed by Spearman correlation analysis.The risk factors of IMT in patients with CKD were analyzed by logistic regression analysis.The receiver operating characteristic(ROC)curve was used to analyze the efficacy of serum miR-210 in the diagnosis of carotid artery atherosclerosis in patients with CKD.Results Compared with the control group,the levels of low density lipoprotein cholesterol(LDL-C),uric acid,serum creatinine,IMT of carotid artery and miR-210 were significantly higher in CKD group,while the levels of Hb,Alb and eGFR were significantly lower in the CKD group(P<0.05).Compared with the normal group and the intima thickening group,the relative expression level of serum miR-210 was significantly increased in the carotid atherosclerotic group(P<0.001).Compared with the normal group,the relative expression level of serum miR-210 in the intima thickening group was significantly increased(P<0.001).There were statistically significant differences in the relative expression levels of serum miR-210 among patients with CKD stage 1,2,3,4 and 5(P<0.001).Spearman correlation analysis showed that the relative expression level of serum miR-210 in CKD patients was significantly positively correlated with IMT of carotid artery,LDL-C and se

关 键 词：慢性肾脏病 微小RNA-210 颈动脉内膜中层厚度 动脉粥样硬化 

分 类 号：R572[医药卫生—消化系统] R447[医药卫生—内科学]