太极拳锻炼对煤工尘肺患者免疫功能的影响  

Effect of Tai Chi Chuan exercise on immune function in patients with coal workers’pneumoconiosis

作　　者：李超[1] 袁宝军[1] 邵仲达 崔晶晶 张淑青[1] LI Chao;YUAN Bao-jun;SHAO Zhong-da;CUI Jing-jing;ZHANG Shu-qing(Department of Clinical Laboratory,Kailuan General Hospital,Tangshan Hebei,063000,China;Department of Anesthesiology,Kailuan Occupational Disease Prevention and Control Hospital,Tangshan Hebei,063000,China)

机构地区：[1]开滦总医院检验科,河北唐山063000 [2]开滦职业病防治院麻醉科,河北唐山063000

出　　处：《职业与健康》2021年第1期16-19,共4页Occupation and Health

摘　　要：目的探讨太极拳锻炼对煤工尘肺(CWP)患者免疫功能的变化影响。方法选取2019年2月1-28日开滦职业病防治院自愿参加太极拳锻炼的35名住院CWP患者,进行为期3个月,每周7次,每次1 h的太极拳锻炼。锻炼开始前1天和锻炼3个月后第2天,采用流式细胞术方法检测CWP患者外周血淋巴细胞亚群CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)、CD19^(+)和CD16^(+)^(+)56^(+)细胞百分比以及调节性CD4^(+)CD25^(+)T细胞、CD19^(+)CD25^(+)B细胞百分比;免疫比浊法检测血清免疫球蛋白(Ig)G、M、A水平和补体CH50、C3及C4水平。结果锻炼后CWP患者外周血CD16^(+)^(+)56^(+)细胞水平较锻炼前升高,差异有统计学意义(P<0.05);CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)、CD19^(+)细胞水平无明显改变(均P>0.05),而CD4^(+)CD25^(+)T细胞和CD19^(+)CD25^(+)B细胞水平及CD4^(+)CD25^(+)/CD4^(+)和CD19^(+)CD25^(+)/CD19^(+)比值较锻炼前显著降低,差异均有统计学意义(均P<0.05);锻炼后血清CH50水平高于锻炼前,差异有统计学意义(P<0.05);血清IgG、IgA、IgM、补体C3和C4水平变化比较,差异均无统计学意义(均P>0.05)。结论太极拳锻炼影响CWP患者外周血淋巴细胞亚群比例分布和补体水平表达,进而影响CWP患者免疫功能。Objective To explore the effect of Tai Chi Chuan exercise on immune function in the patients with coal workers’pneumoconiosis(CWP).Methods Totally 35 CWP inpatients in Kailuan Occupational Disease Prevention and Control Hospital were selected from February 1-28 in 2019,they voluntarily taken the Tai Chi Chuan exercise seven times every week,one hour every time for three months.At one day before exercise and the second day after three months of exercise,the percentages of lymphocytes subsets CD3^(+),CD3^(+)CD4^(+),CD3^(+)CD8^(+),CD19^(+),CD16^(+)^(+)56^(+),the percentages of regulatory CD4^(+)CD25^(+)T cells and CD19^(+)CD25^(+)B cells were determined by a flow cytometer in the peripheral blood collected from CWP patients.The levels of serum immunoglobulin G(IgG),IgM,IgA and CH50,C3,C4 complements were measured using immune-turbidimetric assay.Results After exercise,the levels of peripheral blood CD16^(+)^(+)56^(+)cells significantly increased as compared with before exercise,and the difference was statistically significant(P<0.05).There were no significant differences in the levels of CD3^(+),CD3^(+)CD4^(+),CD3^(+)CD8^(+),and CD19^(+)cells(P>0.05).However,the levels of CD4^(+)CD25^(+)T cells and CD19^(+)CD25^(+)B cells and the ratios of CD4^(+)CD25^(+)/CD4^(+)and CD19^(+)CD25^(+)/CD19^(+)after exercise significantly decreased as compared with before exercise,and the differences were statistically significant(all P<0.05).The level of serum CH50 after exercise was significantly higher than that before exercise(P<0.05),and the difference was statistically significant(P<0.05).There were no significant differences in the levels of serum IgG,IgA,Ig M,C3 and C4(all P>0.05).Conclusion Tai Chi Chuan exercise might affect the proportions distribution of peripheral blood lymphocyte subsets and the levels of complement component expression,and then affect the immune function of CWP patients.

关 键 词：煤工尘肺 太极拳锻炼 淋巴细胞亚群 免疫球蛋白 补体 

分 类 号：R135.2[医药卫生—劳动卫生]