老年帕金森病与肠道菌群、短链脂肪酸和炎性因子相关性研究  

Correlation of Parkinsons disease with gut microbiota,short-chain fatty acids and inflammatory factors in elderly

作　　者：李剑兰 余璇 胡青婷 左丹丹 LI Jian-lan;YU Xuan;HU Qing-ting;ZUO Dan-dan(Neurology Department, Huaian Hospital, Huaian 223200, China)

机构地区：[1]淮安市淮安医院神经内科,江苏省淮安市223200

出　　处：《实用老年医学》2021年第3期282-285,共4页Practical Geriatrics

摘　　要：目的探讨老年PD与肠道菌群、短链脂肪酸和炎性因子的相关性。方法通过16S rDNA基因实时荧光定量PCR检测PD病人和对照组人群粪样普拉梭菌(F.prausnitzii)、直肠真杆菌(E.rectale)、双歧杆菌(Bifidobacterium)、乳酸菌(Lactobacillis)水平;通过气相色谱法检测2组人群粪短链脂肪酸(乙酸、丙酸和丁酸)水平;通过ELISA法检测血清TNF-α、IL-6和IL-10水平,比较2组上述指标的差异。结果与对照组相比,PD病人肠道E.rectale、F.prausnitzii、Lactobacillis、丙酸、丁酸水平均明显降低,差异具有统计学意义(P<0.05)。与对照组相比,PD病人血清TNF-α、IL-6水平明显升高,差异具有统计学意义(P<0.05)。相关分析显示,帕金森综合评分量表总得分与肠道E.rectale、F.prausnitzii、丙酸、丁酸水平呈负相关(P<0.05),与血清TNF-α和IL-6水平呈正相关(P<0.05)。结论老年PD病人肠道产短链脂肪酸细菌水平降低,短链脂肪酸可能通过诱导体内炎症,加剧PD发生发展,但其作用机制还需进一步研究。Objective To investigate the correlation between gut microbiota,short-chain fatty acids and inflammatory factors in the elderly patients with Parkinson's disease(PD).Methods The 16S rDNA gene real-time fluorescent quantitative PCR method was used to detect the levels of F.prausnitzii,E.rectale,Bifidobacterium and Lactobacillis in the feces of PD patients and control group.Gas chromatography was used to detect the levels of short-chain fatty acids(acetic acid,propionic acid and butyric acid)in the intestinal feces and ELISA method was used to detect serum levels of tumor nerosis factor(TNF-α),interloukin-6(IL-6)and IL-10 of the two groups.Results Compared with the control group,the levels of E.rectal,F.prausnitzii,Lactobacillis,propionic acid and butyric acid in the PD group were significantly decreased(P<0.05).Compared with the control group,the plasma levels of TNF-αand IL-6 in the PD group were significantly increased(P<0.05).The total score of the Unified Parkinson's Disease Rating Scale was negatively correlated with the levels of E.rectal,F.prausnitzii propionic acid and butyric acid in intestines(P<0.05),and positively correlated with the serum levels of TNF-αand IL-6(P<0.05).Conclusions In elderly patients with PD,the level of short-chain fatty acid-producing bacteria in the intestines is reduced.Short-chain fatty acids may induce inflammation in the body and aggravate the occurrence and development of PD,but the mechanism needs further study.

关 键 词：帕金森病 肠道菌群 短链脂肪酸 炎症因子 

分 类 号：R749.1[医药卫生—神经病学与精神病学]