Effect of zinc substitution in hydroxyapatite coating on osteoblast and osteoclast differentiation under osteoblast/osteoclast co-culture  

作　　者：Guolong Meng Xiaoli Wu Ruijuan Yao Jing He Wu Yao Fang Wu 

机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,Chengdu 610064,P.R.China

出　　处：《Regenerative Biomaterials》2019年第6期349-359,共11页再生生物材料（英文版）

基　　金：the Natural Science Foundation of China(No.31170922);National Basic Research Program of China(No.2012cb619103);the Research Fund for the Doctoral Program of Higher Education(No.20120181110058);Program for New Century Excellent Talents in University(NCET-12-0387).

摘　　要：Zinc is an essential trace element required for bone remodelling process,but its role in such process remains to be elucidated.In particular,inconsistent results have been reported on the effect of Zn on osteoclastic responses,and supplement of receptor activator of nuclear factor kappa-B ligand(RANKL)factors has been commonly adopted.Co-culture is a suitable approach to elucidating the role of Zn in bone remodelling process,by better imitating the cellular environment as the presence of osteoblasts plays critical role in modulating osteoclastic functions.In this study,zincsubstituted HA coatings have been deposited using a liquid precursor plasma spraying process at two different concentrations(1,2 wt.%).The effect of zinc substitution on osteoblastic and osteoclastic differentiation has been studied in vitro.In particular,a cultivation regime was designed to first induce osteoblastic differentiation of rat bone marrow stromal cells(BMSCs)for 14 days,and then induce osteoclastic differentiation of osteoclast-like precursor RAW 264.7 cells through the aid of the osteoblasts formed for additional 14 days,in the absence of the external addition of RANKL.The results showed that Zn substitution moderately promoted the BMSC differentiation into the osteoblasts and reduced the osteoclastic activity in early time(1 day co-culture).However,promotion of the osteoclastic activity were observed at later stages,as indicated by the significantly enhanced expressions of trap5b and IL-1(8-and 15-day co-culture)and moderate stimulation of the nucleus integration and formation of the multinucleated cells(14-day co-culture).Such stimulating effect of the osteoclastic activity was absent under mono-culture of RAW 264.7 cell,with simple RANKL supplementation.The results suggest that both the zinc and the presence of MSC/osteoblast play profound and highly interacted roles on osteoclast differentiation and activity,which is critical in modulating the bone remodelling process.

关 键 词：ZINC bone marrow stromal cell osteoclast precursor OSTEOCLAST CO-CULTIVATION bone remodelling process 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]