MGMT启动子与垂体腺瘤侵略性行为及ADC值的相关性研究  

Correlation between MGMT promoter and invasive behavior and ADC value of pituitary adenomas

作　　者：张超才 勒德勐杰 林嘉涌 谢仲鹏 韩丽珍[1] 符媚媚 陈峰[1] 黄垂学[1] 王鹏程[1] 赵建农[1] ZHANG Chaocai;LE Demeng-jie;LIN Jia-yong;XIE Zhong-peng;HAN Li-zhen;FU Mei-mei;CHEN Feng;HUANG Chui-xue;WANG Peng-cheng;ZHAO Jian-nong(Department of Neurosurgery,Hainan General Hospital(Hainan Hospital Affiliated to Hainan Medical University),Haikou,570311 China)

机构地区：[1]海南省人民医院/海南医学院附属海南医院神经外科,海南海口570311

出　　处：《解剖学研究》2021年第1期7-11,共5页Anatomy Research

基　　金：海南省社会发展重点项目(ZDYF2019173);海南省自然科学基金青年项目(819QN351)。

摘　　要：目的探讨垂体腺瘤O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)启动子甲基化状态与肿瘤侵略性行为及ADC值的相关性。方法采用前瞻性研究,选取我院2019年01月至2020年08月收治的垂体腺瘤的患者,所有患者术前接受MRI检查(平扫+增强+解剖弥散),术后对肿瘤组织标本进行苏木精-伊红(HE)染色、免疫组化检查,通过焦磷酸测序检测肿瘤MGMT启动子甲基化状态。对比MGMT启动子甲基化状态与垂体腺瘤侵袭性分组,功能分型,增值性分组以及ADC值之间的相关性。结果17例增值组患者中有2例患者伴有MGMT突变,MGMT无突变患者15例,MGMT启动子甲基化突变状态与肿瘤的增值性存在明显相关(P<0.05)。30例影像学侵袭组患者中,8例患者有MGMT突变,MGMT无突变患者22例,两组差异无统计学意义(P>0.05)。21功能型垂体腺瘤患者中,有7例患者有MGMT突变,MGMT无突变患者14例,两组间差异无统计学意义(P>0.05)。此外,18例MGMT甲基化突变组的垂体腺瘤ADC平均值为844.67,37例MGMT无突变组ADC平均值为791.14,差异无统计学意义(P>0.05)。结论垂体腺瘤MGMT启动子甲基化状态与Ki-67表达密切相关,有望成为预判垂体腺瘤侵略性行为的重要指标。Objective To investigate the correlation between MGMT promoter methylation status and tumor aggres-sive behavior and ADC value in pituitary adenomas.MethodsA prospective study was conducted to select patients with pi-tuitary adenomas in our hospital from January 2019 to August 2020.All patients underwent MRI examination(plain scan+enhancement+anatomic diffusion)before operation.After operation,tumor tissue samples were examined by HE stainingand immunohistochemistry.The methylation status of MGMT promoter was detected by pyrophosphate sequence.The correla-tion between MGMT promoter methylation status and invasive group,functional group,value-added group and ADC value ofpituitary adenomas was compared.Results There were 2 patients with MGMT mutation and 15 patients without MGMT muta-tion in the 17 cases of value-added group.There was a significant correlation between MGMT promoter methylation mutationand tumor value-added(P<0.05).There were 8 patients with MGMT mutation and 22 patients without MGMT mutation inthe imaging invasion group.There was no significant difference between the two groups(P>0.05).There were 7 patients withMGMT mutation and 14 patients without MGMT mutation in 21 functional pituitary adenomas.There was no significant differ-ence between the two groups(P>0.05).In addition,the average ADC value of pituitary adenomas in 18 cases of MGMTmethylation mutation group was 844.67,and that in 37 cases of MGMT non mutation group was 791.14,with no significantdifference(P>0.05).Conclusion The methylation status of MGMT promoter in pituitary adenomas is closely related to theexpression of Ki-67,which is expected to be an important predictor of invasive behavior in pituitary adenomas.

关 键 词：垂体腺瘤 O-6-甲基鸟嘌呤DNA甲基转移酶启动子 解剖弥散成像 侵略性行为 预后 

分 类 号：R736.4[医药卫生—肿瘤]