LncRNA-MEG3和KLF4在鼻咽癌发生发展中的作用及可能机制  

Role and Possible Mechanism of LncRNA-MEG3 and KLF4 in Carcinogenesis and Progression of Nasopharyngeal Carcinoma

作　　者：宁佳羽 包伟晶[2] 周素娟 魏日富[2] 朱忠寿[2] NING Jiayu;BAO Weijing;ZHOU Sujun;WEI Rifu;ZHU Zhongshou(Department of Pathology,Ningde Municipal Hospital,Ningde 352100,China;Department of Otolaryngology&Head and Neck Surgery,Ningde Municipal Hospital,Ningde 352100,China)

机构地区：[1]宁德市医院病理科,宁德352100 [2]宁德市医院耳鼻咽喉头颈外科,宁德352100

出　　处：《肿瘤防治研究》2021年第3期234-238,共5页Cancer Research on Prevention and Treatment

基　　金：福建省自然科学基金(2018J01226,2019J01628)。

摘　　要：目的探讨LncRNAs-MEG3在鼻咽癌发生发展中的作用及其可能的分子机制。方法qRTPCR检测鼻咽癌细胞中MEG3与miR-543的含量,荧光素酶报告法研究MEG3与miR-543的关系,分析MEG3和KLF4介导的鼻咽癌细胞增殖和凋亡的变化,Western blot检测KLF4、Bcl-2和Bax蛋白表达水平。结果鼻咽癌细胞株中MEG3的表达呈下降趋势,miR-543的表达呈上升趋势,与对照组相比差异有统计学意义(P<0.05);荧光素酶报告和Western blot显示MEG3可结合miR-543以调控KLF4的表达,进而抑制肿瘤细胞增殖、促进肿瘤细胞凋亡并影响Bcl-2和Bax蛋白表达水平,与对照组相比差异有统计学意义(P<0.05)。结论LncRNA-MEG3可通过吸附结合miR-543靶向调控KLF4的表达,发挥抑制鼻咽癌发生发展的作用。LncRNA-MEG3可能是鼻咽癌靶向治疗的一种新的生物标志物。Objective To investigate the role of LncRNAs-MEG3 in the carcinogenesis and progression of nasopharyngeal carcinoma(NPC)and the possible molecular mechanism.Methods qRT-PCR was used to detect the content of MEG3 and miR-543 in NPC cells.Luciferase reporter method was used to study the relation between MEG3 and miR-543,and the changes of cell proliferation and apoptosis induced by MEG3 or KLF4 were analyzed.Western blot was used to detect the expression of KLF4,Bcl-2 and Bax proteins.Results Compared with the control group,the expression of miR-543 in NPC cell line was significantly increased(P<0.05),while the expression of MEG3 was decreased(P<0.05).Luciferase report and Western blot showed that MEG3 could regulate the expression of KLF4 by adsorbing miR-543 to inhibit cell proliferation,promote cell apoptosis and affect the expression levels of Bcl-2 and Bax proteins.Conclusion LncRNA-MEG3 could regulate the expression of KLF4 by adsorbing miR-543 and then plays a role in inhibiting the occurrence and development of NPC.It may be a new biomarker for NPC targeted therapy.

关 键 词：鼻咽癌细胞 母系表达基因3 miR-543 Krüppel样因子4 

分 类 号：R739.63[医药卫生—肿瘤]