食管鳞癌中LncRNA DLEU1的表达及对食管鳞癌细胞增殖和迁移的影响  

Expression of LncRNA DLEU1 in Esophageal Squamous Cell Carcinoma and Its Effect on Proliferation and Migration of ESCC Cells

作　　者：段玉青[1] 王郁[1] 王雪晓 刘丽华[1,2] DUAN Yuqing;WANG Yu;WANG Xuexiao;LIU Lihua(Department of Tumor Immunology,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050035,China;Hebei Cancer Institute,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院肿瘤免疫科,石家庄050035 [2]河北省肿瘤研究所,石家庄050011

出　　处：《肿瘤防治研究》2021年第3期255-260,共6页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(81871894,91942314);省部共建食管癌防治国家重点实验室开放课题(K2020-004)。

摘　　要：目的研究长链非编码RNA淋巴细胞白血病缺失基因1(DLEU1)在食管鳞状细胞癌(ESCC)组织中的表达,及其对肿瘤细胞增殖和迁移的影响。方法收集58例手术切除的ESCC及其相应癌旁组织,RT-qPCR检测ESCC组织和细胞中DLEU1的表达水平。Log rank Test法分析ESCC组织中DLEU1的表达与患者临床病理特征的关系。Kaplan-Meier法分析DLEU1表达与ESCC患者生存的相关性。多变量Cox回归模型评估DLEU1对ESCC患者预后的影响。采用CCK-8和划痕愈合实验检测DLEU1对Eca9706细胞增殖和迁移的影响。结果ESCC组织中DLEU1高表达(P<0.01),其高表达与ESCC患者肿瘤大小、TNM分期和淋巴结转移呈显著相关性(均P<0.05)。DLEU1高表达与ESCC患者预后不良呈负相关(P<0.01),且是影响ESCC患者预后的独立危险因素(P<0.05)。与对照组相比,体外敲低DLEU1的表达可显著抑制Eca9706细胞的增殖和迁移能力(P<0.01)。结论DLEU1在ESCC组织中高表达,是影响ESCC患者预后的独立危险因素,且具有促进肿瘤细胞增殖和迁移的能力。Objective To investigate the expression of LncRNA DLEU1 in ESCC tissues,and its effect on the proliferation and migration of ESCC cells.Methods We collected 58 cases of ESCC tissues and corresponding para-cancerous tissues.RT-qPCR was used to detect the relative expression levels of DLEU1 in ESCC tissues and cells.Log-rank test was used to analyze the relation between the expression of DLEU1 and clinicopathological features.Kaplan-Meier analysis was used to investigate the correlation between the expression of DLEU1 and the survival of ESCC patients.Multivariate Cox regression model was used to evaluate the effect of DLEU1 on the prognosis of ESCC patients.Effects of DLEU1 on the proliferation and migration of Eca9706 cells were evaluated by CCK-8 and wound healing assays,respectively.Results DLEU1 was highly expressed in ESCC tissues(P<0.01)and significantly correlated with tumor size,TNM stage and lymph node metastasis(all P<0.05).High expression of DLEU1 was negatively correlated with poor prognosis of ESCC patients(P<0.01),and DLEU1 was also an independent prognostic risk factor(P<0.05).Moreover,knockdown of DLEU1 significantly inhibited the proliferation and migration of Eca9706 cells,compared with the control group(P<0.01).Conclusion DLEU1 is highly expressed in ESCC tissues.The expression of DLEU1 is an independent risk factor for the prognosis of ESCC patients and promotes ESCC cell proliferation and migration.

关 键 词：食管鳞状细胞癌 长链非编码RNA 淋巴细胞白血病缺失基因1 临床病理特征 增殖 迁移 

分 类 号：R735.1[医药卫生—肿瘤] R730.2[医药卫生—临床医学]