异硫氰酸苯乙酯联合阿霉素诱导骨肉瘤细胞凋亡的作用研究  

A study on the apoptosis of osteosarcoma cells induced by phenethyl isothiocyanate combined with adriamycin

作　　者：范锲 李文浩 丘德赞[1] 黄孝英[1] 李浩曦 何基琛 FAN Qie;LI Wen-hao;QIU De-zan(Department of Spine and Bone Diseases, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China)

机构地区：[1]广西壮族自治区人民医院脊柱骨病外科,南宁530021

出　　处：《中国临床新医学》2021年第3期287-292,共6页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西卫健委科研课题(编号:Z2016632)。

摘　　要：目的探讨异硫氰酸苯乙酯(PEITC)联合阿霉素(ADM)诱导骨肉瘤细胞凋亡的作用。方法选用U2-OS细胞株,根据处理方法的不同将其分为PEITC组、ADM组、PEITC+ADM组和空白对照组。采用MTT法检测不同处理组药物对U2-OS细胞增殖能力的影响。采用TUNEL法测定不同处理组U2-OS细胞的凋亡情况。采用Western blot法检测不同处理组U2-OS细胞Caspase-3、Fas、FasL蛋白的表达情况。结果PEITC和ADM浓度对U2-OS细胞的半抑制浓度(IC50)分别为4.37μM/ml和6.61μg/ml。与单独使用PEITC或ADM处理相比,联合使用两种药物对U2-OS细胞的增殖抑制率更高(P<0.05)。低剂量的PEITC联合ADM产生协同效应,而高剂量的两种药物联合产生相加效应。与单药物处理相比,PEITC联合ADM能够显著提高U2-OS细胞凋亡率(P<0.05),增加Caspase-3蛋白的活性及表达(P<0.05)。结论PEITC增强了ADM诱导骨肉瘤细胞凋亡的作用,可能与Caspase-3蛋白活性升高和表达量上调有关,这为PEITC联合ADM的临床应用提供了参考依据。Objective To explore the effect of phenethyl isothiocyanate(PEITC)combined with adriamycin(ADM)on the apoptosis of osteosarcoma cells.Methods Osteosarcoma U2-OS cell line was selected and the cells were divided into PEITC group,ADM group,PEITC+ADM group and blank control group according to different treatment methods.Methyl thiazolyl tetrazolium(MTT)method was used to detect the drug effects of different treatment groups on the proliferation of U2-OS cells.Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL)method was used to determine the apoptosis of U2-OS cells in different treatment groups.Western blot was used to detect the expressions of Caspase-3,Fas and FasL proteins in U2-OS cells in different treatment groups.Results 50%inhibiting concentration(IC50)of PEITC and ADM on U2-OS cells were 4.37μM and 6.61μg/ml,respectively.Compared with PEITC or ADM treatment alone,the combined use of the two drugs had a higher proliferation inhibitory rate on U2-OS cells(P<0.05).Low-dose PEITC combined with ADM produced a synergistic effect,while high-dose PEITC combined with ADM produced an additive effect.Compared with single-drug treatment,PEITC combined with ADM could significantly increase the apoptosis rate of U2-OS cells(P<0.05),and increased the activity and expression of Caspase-3 protein(P<0.05).Conclusion PEITC enhances the apoptosis of osteosarcoma cells induced by ADM,which may be related to the increased activity and up-regulation of Caspase-3 protein.This provides a reference for the clinical application of PEITC combined with ADM.

关 键 词：阿霉素 异硫氰酸苯乙酯 骨肉瘤 凋亡 

分 类 号：R738[医药卫生—肿瘤]