超声微泡联合骨髓间充质干细胞移植对脊髓损伤大鼠的神经保护作用研究  

The Neuroprotective Effect of Ultrasound Microbubbles Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on Spinal Cord Injury Rats

作　　者：陈宇[1] 张智[1] 汪凡栋[1] 宋昭君 刘元彬[1] 唐龙[1] 廖伟[1] 郑佳状[1] CHEN Yu;ZHANG Zhi;WANG Fandong;SONG Zhaojun;LIU Yuanbin;TANG Long;LIAO Wei;ZHENG Jiazhuang(Spine Surgery,Suining Central Hospital Suining,Sichuan 629000,China)

机构地区：[1]四川遂宁市中心医院脊柱外科,四川遂宁629000

出　　处：《中国中医骨伤科杂志》2021年第3期1-6,共6页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：四川省卫生计生委科研课题(16PJ523)。

摘　　要：目的:探讨超声微泡联合骨髓间充质干细胞(BMSCs)移植对脊髓损伤大鼠的神经保护作用。方法:采用Allen’s打击法构建SD大鼠脊髓损伤模型并分组进行对应处理,术后进行BBB评分,HE染色和尼氏染色检测脊髓组织与尼氏小体形态变化,ELISA检测炎症因子水平,免疫荧光染色和qRT-PCR检测神经相关因子与凋亡相关因子表达。结果:与模型组比较,超声微泡组、BMSCs组和超声微泡+BMSCs组术后第7天和第21天BBB评分增加,脊髓组织病理损伤减轻,尼氏小体形态得到改善,炎症反应减轻,Nestin,GFAP表达及BDNF,NT3,Bcl-2 mRNA表达升高,Bax,Caspase-3 mRNA表达下降,差异有统计学意义(P<0.05),且超声微泡和BMSCs联用效果更佳。结论:超声微泡联合BMSCs移植能够对脊髓损伤大鼠发挥神经保护的作用。Objective:To investigate the neuroprotective effect of ultrasound microbubbles combined with bone marrow mesenchymal stem cells(BMSCs)transplantation on spinal cord injury rats.Methods:The SD rat spinal cord injury model was constructed by Allen’s strike method and divided into groups for corresponding treatment.After the operation BBB score was performed.HE staining and Nissl staining were used to detect the morphological changes of spinal cord tissue and Nissl body.ELISA was used to detect inflammatory factor levels,and immunofluorescence staining and qRT-PCR detects the expression of nerve-related factors and apoptosis-related factors.Results:Compared with the model group,the BBB scores in the ultrasound microbubble group,BMSCs group and ultrasound microbubble+BMSCs group increased on the 7 th and 21 th day after operation.The pathological damage of the spinal cord tissue was reduced,and the morphology of Nissl bodies was improved.Inflammation was reduced,Nestin,GFAP expression and BDNF,NT3,Bcl-2 mRNA expression increased,Bax and Caspase-3 mRNA expression decreased,and the combined effect of ultrasound microbubbles and BMSCs was better.Conclusion:Ultrasound microbubble combined with BMSCs transplantation exerts neuroprotective effect on spinal cord injury rats.

关 键 词：超声微泡 骨髓间充质干细胞 脊髓损伤 神经保护 炎症反应 

分 类 号：R-33[医药卫生]