抗菌肽GH12对龋相关三菌种生物膜形貌及菌种构成的影响  

Effects of antimicrobial peptide GH12 on the morphology and composition of cariogenic three-species biofilm

作　　者：李欣蔚 王雨霏 姜文韬 张凌琳[1] Li Xinwei;Wang Yufei;Jiang Wentao;Zhang Linglin(State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Dept.of Cariology and Endodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China)

机构地区：[1]口腔疾病研究国家重点实验室国家口腔疾病临床医学研究中心四川大学华西口腔医院牙体牙髓病科,成都610041

出　　处：《华西口腔医学杂志》2021年第2期188-194,共7页West China Journal of Stomatology

基　　金：国家自然科学基金面上项目(81771062);四川省青年科技创新研究团队项目(2020JDTD0014)。

摘　　要：目的研究抗菌肽GH12对龋相关三菌种生物膜结构、形貌以及菌种构成的影响。方法龋相关三菌种生物膜含有致龋菌变异链球菌(S.mutans)以及共生菌血链球菌(S.sanguinis)和格式链球菌(S.gordonii)。以未处理组作为对照组,GH12(2、4、8 mg·L^(-1))作为实验组。通过结晶紫染色、扫描电镜和荧光原位杂交观察生物膜形貌变化,定性分析菌种构成,继而使用选择培养计数法定量分析生物膜内的S.mutans占比。结果与对照组相比,GH12处理后的龋相关三菌种生物膜生物量减少,密度降低,结构更松散;S.mutans占比逐渐降低甚至无法检测,而S.gordonii和S.sanguinis占比逐渐增加,占据主导地位。结论GH12可以降低龋相关三菌种生物膜中致龋菌占比,破坏其完整性,使其向更易清除的方向转换。Objective To evaluate the effects of antimicrobial peptide GH12 designed de novo on the structure,morphology,and composition of a cariogenic three-species biofilm.Methods The cariogenic three-species biofilm consisted of the cariogenic Streptococcus mutans(S.mutans)and commensal bacteria Streptococcus sanguinis(S.sanguinis)and Streptococcus gordonii(S.gordonii).The biofilm was treated using GH12(2,4,and 8 mg·L-1),and untreated biofilm was used as the control.Changes in the morphology and structure of the three-species biofilm were evaluated through crystal violet staining,scanning electron microscopy(SEM),and fluorescent in situ hybridization(FISH).Moreover,S.mutans in the biofilm was selectively cultured,and its colony-forming units were counted.Results The biomass and density of the cariogenic three-species biofilm treated with GH12 decreased compared with those of the control.The number of S.mutans decreased gradually and eventually became undetectable,whereas the number of S.gordonii and S.sanguinis increased and became predominant in the biofilm.Conclusion GH12 can reduce the number of S.mutans within the cariogenic three-species biofilm,destroys its integrity,and consequently makes the biofilm easy to remove.

关 键 词：抗菌肽 牙菌斑 变异链球菌 血链球菌 格氏链球菌 

分 类 号：R781.1[医药卫生—口腔医学]