BoneCeramic和Bio-Oss骨粉对狗骨髓间充质干细胞体外成骨分化能力影响的比较研究  

A comparative study of effects of Boneceramic and Bio-Oss on osteogenic differentiation of dog bone marrow mesenchymal stem cells in vitro

作　　者：路佳佳 李伟琼 许敏[1] 徐涛[2] 朱友明 徐建光 张红艳[1] LU Jia-jia;LI Wei-qiong;XU Min;XU Tao;ZHU You-ming;XU Jian-guang;ZHANG Hong-yan(School of Stomatology,Anhui Medical University,Laboratory of Anhui Oral Disease Research Center,Hefei 230032,China;College of Pharmacy,Anhui Medical University,Hefei 230032,China)

机构地区：[1]安徽医科大学附属口腔医学院,安徽省口腔疾病研究中心实验室 [2]安徽医科大学药学院,安徽合肥230032

出　　处：《中国药理学通报》2021年第4期511-516,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 31970677)。

摘　　要：目的比较BoneCeramic和Bio-Oss骨粉对狗骨髓间充质干细胞(DBMSCs)的增殖、黏附和体外成骨分化能力的影响。方法体外分离培养DBMSCs并进行成骨、成脂分化鉴定;CCK-8检测BoneCeramic和Bio-Oss两种骨粉浸提原液对DBMSCs活力的影响;SEM观察细胞在骨粉表面的黏附情况;ALP定量检测细胞在骨粉表面的成骨分化能力,RT-PCR检测OPN、OCN、RUNX-2和ALP基因的相对表达情况。结果从狗骨髓中分离培养的细胞符合间充质干细胞的特征;CCK-8结果显示两种骨粉浸提液均对DBMSCs没有明显的细胞毒性作用;SEM结果显示,与Bio-Oss相比,DBMSCs在BoneCeramic的表面分布更广;ALP定量检测显示DBMSCs在BoneCeramic表面比Bio-Oss更有利于其成骨分化,同时使OPN、OCN、RUNX-2和ALP基因的表达升高。结论体外研究表明,Bio-Oss和BoneCeramic均具有良好的生物相容性,与Bio-Oss骨粉相比,BoneCeramic更加有利于DBMSCs的成骨分化。Aim To compare the effects of BoneCeramic and Bio-Oss on the proliferation,adhesion and osteogenic differentiation of dog bone marrow mesenchymal stem cells(DBMSCs)in vitro.Methods DBMSCs were isolated and cultured in vitro and identified for osteogenic and adipogenic differentiation.CCK-8 was used to detect the effects of BoneCeramic and Bio-Oss bone meal extracts on the vitality of DBMSCs.SEM was used to observe the adhesion of DBMSCs on the surface of bone meal.ALP was used to quantitatively detect the osteogenic differentiation of DBMSCs on the surface of bone meal,and the relative expressions of OPN,OCN,RUNX-2,and ALP genes were detected by RT-PCR.Results The cells isolated and cultured from dog bone marrow conformed to the characteristics of mesenchymal stem cells.CCK-8 results showed that the stock solutions of Bio-Oss and Bone Ceramic had no obvious cytotoxic effect on DBMSCs.SEM showed that DBMSCs were distributed more widely on the surface of BoneCeramic than that of Bio-Oss.ALP quantitative detection showed that DBMSCs on the BoneCeramic surface were more conducive to its osteogenic differentiation than Bio-Oss,and it also increased the expression of OPN,OCN,RUNX-2 and ALP genes.Conclusions In vitro studies show that both Bio-Oss and BoneCeramic have good biocompatibility.Compared with Bio-Oss bone meal,BoneCeramic is more conducive to the osteogenic differentiation of DBMSCs.

关 键 词：BoneCeramic BIO-OSS 骨移植 骨髓间充质干细胞 成骨分化 生物相容性 

分 类 号：R-332[医药卫生] R329.24