新型循环肿瘤细胞富集及精准分析方法的研究进展  

Advance in Novel Methods for Enrichment and PreciseAnalysis of Circulating Tumor Cells

作　　者：申聪聪 吴呈珂[1] 陈粤华[1] 王建秀[2] 阳明辉[2] 仉华 SHEN Cong-Cong;WU Cheng-Ke;CHEN Yue-Hua;WANG Jian-Xiu;YANG Ming-Hui;ZHANG Hua(College of Chemistry and Chemical Engineering,Henan Normal University,Xinxiang 453007,China;College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China)

机构地区：[1]河南师范大学化学化工学院,新乡453007 [2]中南大学化学化工学院,长沙410083

出　　处：《分析化学》2021年第4期483-495,共13页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.22004028,21722501,21803018);河南省高层次人才特殊支持项目(No.204200510006);河南省重点科技攻关计划项目(Nos.202102310139,202102311071)资助。

摘　　要：外周血中循环肿瘤细胞(Circulating tumor cell,CTC)是癌症转移的重要媒介,可提供完整的细胞生物学信息,被认为是目前最具有应用前景的液体活检靶标之一。然而,全血中CTC具有含量极低、基质环境复杂、自身异质性强等特点,因此,开发高效的富集分离及检测方法是实现CTC精准分析的关键。目前已报道的CTC分析方法主要是基于CTC的物理或生化特性,将传统的密度梯度离心、超微膜过滤、免疫磁分离结合新型的微流控、双向电泳-场分离等技术,再联合免疫细胞化学、光声成像、表面增强拉曼散射、电化学法等方法,实现CTC的精准分析。本文对近年来CTC的富集分离技术、检测方法、临床检测面临的挑战等进行了探讨和总结,以期为进一步完善CTC的富集分离、精准计数及分子表型等方面的基础研究及临床应用提供参考。The dissemination of circulating tumor cells(CTCs)in peripheral blood is an important intermediate step for cancer metastasis.CTC can provide complete information of cell biology and thus has become one of the most promising targets of“liquid biopsy”.However,due to the extremely rarity,complex environment and strong heterogeneity of CTC in peripheral blood,the development of efficient enrichment and separation methods are the key in the accurate detection of CTC.Current reported CTC enrichment and analysis techniques are mainly based on the physical or biological characteristics of CTC.These methods are commonly combined with the traditional techniques including density gradient centrifugation,superfine membrane filtration and immunomagnetic separation,the novel methods such as microfluidic chip separation and two-dimensional electrophoresis-field separation,and the analysis techniques such as reverse transcription-polymerase chain reaction,flow cytometry,immunocytochemistry,photoacoustic imaging,surface-enhancement Raman scattering and electrochemistry to realize the accurate detection of CTC.In this review,the recent progress on enrichment techniques and detection methods for CTC was summarized,also the challenge and their clinical application status were discussed,which could provide reference for further improvement of the technology of enrichment,accurate counting and molecular phenotype analysis for CTC,as well as the application in scientific research and clinical practice.

关 键 词：循环肿瘤细胞 富集分离 精准分析 液体活检 癌症转移 评述 

分 类 号：R73-3[医药卫生—肿瘤]