Siva-1在胃癌组织中的表达及其临床意义  

Expression and clinical significance of Siva-1 in gastric cancer

作　　者：孔凡彪 邓洪强[1] 徐胜[1] 王晓通[2] 麦威[2] 庞黎明 Fan-biao Kong;Hong-qiang Deng;Sheng Xu;Xiao-tong Wang;Wei Mai;Li-ming Pang(Department of Colorectal and Anal Surgery,People's Hospital of Guangxi Zhuang Autonomous Region,Nanning,Guangxi 530023,China;Department of Surgical Treatment of Gastrointestinal Hernia and Fistula,People's Hospital of Guangxi Zhuang Autonomous Region,Nanning,Guangxi 530023,China)

机构地区：[1]广西壮族自治区人民医院结直肠肛门外科,广西南宁530023 [2]广西壮族自治区人民医院胃肠疝肠瘘外科,广西南宁530023

出　　处：《中国现代医学杂志》2021年第6期1-4,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81660416);广西卫生健康委自筹课题(No:Z20180739);广西壮族自治区人民医院青年基金(No:QN2018-22)。

摘　　要：目的探讨Siva-1在胃癌组织中的表达及其与细胞凋亡的关系。方法选取2016年1月—2018年12月广西壮族自治区人民医院经手术切除的胃癌组织及癌旁组织各54例。采用qRT-PCR和SP法检测Siva-1mRNA及蛋白的表达水平,分析Siva-1在胃癌组织中的表达及与临床病理特征的关系。结果胃癌组织中Siva-1阳性表达率较低(P <0.05),Siva-1 m RNA相对表达量较低(P <0.05)。不同肿瘤直径、肿瘤分期患者Siva-1阳性表达率比较,差异有统计学意义(P <0.05)。经Pearson相关性分析,Siva-1 mRNA表达量与肿瘤直径呈负相关(r=-0.376,P <0.05)。结论 Siva-1在胃癌组织中低表达,且与肿瘤的发生、发展有关,可作为诊断胃癌的潜在标志物。Objective To detect the expression of Siva-1 in gastric cancer tissue, and to investigate the relationship between Siva-1 expression level and the clinical characteristics of gastric cancer. Methods We selected the gastric cancer tissues and adjacent normal tissues surgically removed in our hospital from January 2016 to December 2018, each with 54 cases. Quantitative real-time polymerase chain reaction(qRT-PCR) and immunohistochemistry were used to detect the mRNA and protein expression of Siva-1 in the gastric cancer and corresponding adjacent normal tissues. The relationship between the expression of Siva-1 and the clinicopathological features was analyzed. Results The expression level of Siva-1 in gastric cancer tissues was significantly lower than that in adjacent normal tissues(P < 0.05). The positive rate of Siva-1 differed in tissues with different tumor diameter and stage(P < 0.05). The correlation analysis further suggested Siva-1 expression negatively correlated with tumor size and pathological grade(P < 0.05). Conclusions Siva-1 is lowly expressed in gastric cancer tissues,and is negatively associated with tumor size and pathological grade, indicating that Siva-1 may play a role in suppressing gastric cancer as a potential biomarker.

关 键 词：胃肿瘤 Siva-1/凋亡诱导因子 肿瘤标记 生物学 

分 类 号：R735.2[医药卫生—肿瘤]