Preosteoblast-enriched lnc-Evf2 facilitates osteogenic differentiation by targeting Notch  

作　　者：Zhen Zhang Haixia Qi Han Xia Qi Liu Yi Ren Kun Zhang Yuan Xue Wei Hong 

机构地区：[1]Department of Histology and Embryology,School of Basic Medical Sciences,Tianjin Medical University,Tianjin 300070,China [2]Department of Orthopedic Surgery,Tianjin Key Laboratory of Spine and Spinal Cord,Tianjin Medical University General Hospital,Tianjin 300070,China [3]Department of Emergency Medicine,Tianjin Medical University General Hospital,Tianjin Medical University,Tianjin 300070,China

出　　处：《Acta Biochimica et Biophysica Sinica》2021年第2期179-188,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(Nos.81971331,81871124,and 81800542);the Natural Science Foundation of Tianjin(Nos.18JCZDJC99000 and 18JCQNJC81500);the Science&c Technology Development Fund of Tianjin Education Commission for Higher Education(No.2019KJ170).

摘　　要：Ossification of ligaments(OL)and osteoporosis(OP)are multifactorial disorders without definitive clinical biomarkers.Long non-coding RNAs(lncRNAs)are known to involve in regulating pathogenesis.Here,we have identified a preosteoblast-enriched lnc-Evf2 that was overexpressed in ossified ligamentum flavum(OLF)and down-expressed in OP.lnc-Evf2 is gradually upregulated during osteogenic induction,correlating with the enhanced expression of osteogenic marker genes and matrix mineralization.Moreover,knockdown of lnc-Evf2 significantly inhibits the expression of osteogenic differentiation markers and delays the osteoblastic mineralization process,indicating that this molecule is involved in osteogenesis.Mechanistically,we demonstrated that silencing of lnc-Evf2 decreases the protein level but not the mRNA levels of Notch2,Notch3,and Hes1,all of which correlate with osteogenesis.Taken together,our data demonstrate that lnc-Evf2 promotes osteogenic differentiation and bone formation through the Notch signaling,revealing that lnc-Evf2 may serve as a novel potential clinical target of OL and OP.

关 键 词：osteogenic differentiation lncRNA ossification of ligaments OSTEOPOROSIS NOTCH 

分 类 号：R681[医药卫生—骨科学]