长链非编码RNA与mRNA在急性髓性白血病中的异常表达及其功能初探  

Expressions and activities of lncRNAs and mRNAs in acute myeloid leukemia

作　　者：田薇[1,2] 方延[2] 吴晗恬 吉布强 夏昭林[2] TIAN Wei;FANG Yan;WU Hantian;JI Buqiang;XIA Zhaolin(Public Health College,Xinjiang Medical University,Urumqi 830011,Xinjiang;Public Health College,Fudan University,Shanghai 200032;Department of Hematology,Linyi People's Hospital,Linyi 276100,Shandong,China)

机构地区：[1]新疆医科大学公共卫生学院,新疆乌鲁木齐830011 [2]复旦大学公共卫生学院,上海200032 [3]山东省临沂市人民医院血液科,山东临沂276100

出　　处：《癌变．畸变．突变》2021年第2期110-115,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：新疆维吾尔自治区高校科研计划项目(XJEDU2020Y023)。

摘　　要：目的:探讨急性髓性白血病患者和健康对照人群中长链非编码RNA(lncRNA)和mRNA的差异表达,初步建立急性髓性白血病患者lncRNA异常表达谱。方法:采用Human lncRNA芯片,对急性髓性白血病患者及健康对照人群外周静脉血各6例共12个标本进行lncRNAs及mRNAs检测,并采用基因本体(GO)分析和KEGG通路初步分析差异表达基因的生物学功能。结果:与健康对照组相比,急性髓性白血病组患者差异表达的lncRNA有3256个,其中1161个表达上调,2095个表达下调;差异表达的mRNA有3544个,其中1950个表达上调,1594个表达下调;GO分析表明,差异表达基因功能注释主要包括化学刺激感受、有丝分裂细胞周期的G1/S转变、炎症反应等。KEGG分析表明,差异表达基因主要富集在移植物抗宿主反应疾病、细胞循环及人类嗜T淋巴细胞病毒I型感染等通路上。结论:本研究筛选出急性髓性白血病患者异常表达的部分lncRNA和mRNA,为深入研究白血病发病分子机制提供了理论依据。OBJECTIVE:To explore the differential expression and activities of lncRNAs and mRNAs in lymphocytes from acute myeloid leukemia patients compared with that from normal controls.METHODS:Microarrays were used to detect discrepant lncRNAs and mRNAs in peripheral lymphocytes from 6 acute myeloid leukemia patients compared with 6 normal controls.GO and KEGG were used to analyze the primary biological functions of differentially expressed genes.RESULTS:In lymphocytes from the two groups of individuals,there were 3256 differentially expressed lncRNAs between patients and controls.Among these lncRNAs,1161 lncRNAs were upregulated and 2059 were downregulated.In addition,there were 3544 differentially expressed mRNAs between patients and controls;1950 mRNA were upregulated and 1594 were downregulated.GO analyses show that functional annotation of the differential expression genes mainly included detection of chemical stimulus involved in sensory perception of smell,G1/S transition of mitotic cell cycle and inflammatory response.The graft-versus-host disease and cell cycle,HTLV-I infection and other pathways were shown by KEGG.CONCLUSION:These findings extended the current knowledge and provided some novel mechanisms on pathogenesis of leukemia.

关 键 词：急性髓性白血病 长链非编码RNA MRNA 基因芯片 

分 类 号：R733.7[医药卫生—肿瘤]