DnaJA4 is involved in responses to hyperthermia by regulating the expression of F-actin in HaCaT cells  

作　　者：Rui-Jiao Liu Xue-Li Niu Jin-Ping Yuan Hong-Duo Chen Xing-Hua Gao Rui-Qun Qi 

机构地区：[1]Department of Dermatology,The First Hospital of China Medical University,Shenyang,Liaoning 110001,China [2]National Health Commission Key Laboratory of Immunodermatology(China Medical University),Shenyang,Liaoning 110001,China [3]Key Laboratory of Immunodermatology(China Medical University),Ministry of Education,Shenyang,Liaoning 110001,China [4]National and Local Joint Engineering Research Center of Immunodermatological Theranostics,Shenyang,Liaoning 110001,China.

出　　处：《Chinese Medical Journal》2021年第4期456-462,共7页中华医学杂志（英文版）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China(No.81673070)。

摘　　要：Background:Hyperthermia in combination with DnaJA4-knockout(KO)obviously affects the anti-viral immunity of HaCaT cells.The mechanisms of this process are not yet fully explored.However,it is known that DnaJA4 interacts with actin cytoskeleton after hyperthermia.Our aim was to investigate the effects of DnaJA4 on F-actin in HaCaT cells following hyperthermia.Methods:Wild-type(WT)and DnaJA4-KO HaCaT cells were isolated at either 37°C(unheated)or 44°C(hyperthermia)for 30 min followed by testing under conditions of 37°C and assessing at 6,12,and 24 h after hyperthermia.The cytoskeleton was observed with immunofluorescence.Flow cytometry and Western blotting were used to detect the expression of F-actin and relevant pathway protein.Results:DnaJA4-KO and hyperthermia changed the cytoskeleton morphology of HaCaT cells.F-actin expression levels were elevated in DnaJA4-KO cells compared with WT cells(6364.33±989.10 vs.4272.67±918.50,P<0.05).In response to hyperthermia,F-actin expression levels of both WT and DnaJA4-KO cells showed a tendency to decrease followed by an obvious recovery after hyperthermia(WT cells:unheated vs.6 h after hyperthermia or 24 h after hyperthermia:0.34±0.02 vs.0.24±0.01,0.31±0.01,P<0.001,P<0.05;DnaJA4-KO cells:unheated vs.6 h after hyperthermia or 24 h after hyperthermia:0.44±0.01 vs.0.30±0.01,0.51±0.02,P<0.001,P<0.01).WT cells restored to baseline levels observed in the unheated condition,while DnaJA4-KO cells exceeded baseline levels in the recovery.As the upstream factors of F-actin,a similar profile in rho-associated serine/threonine kinase 1(ROCK 1)and RhoA expressions was observed after hyperthermia.While E-cadherin expression was decreased in response to hyperthermia,it was increased in DnaJA4-KO cells compared with WT cells.Conclusions:Hyperthermia affects the expression levels of F-actin in HaCaT cells.DnaJA4 knockout increases the expression of F-actin in HaCaT cells after hyperthermia.DnaJA4 regulates the expressions of F-actin and the related pathway proteins in response t

关 键 词：HYPERTHERMIA DnaJA4 F-ACTIN HACAT 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]