食源性病毒核酸恒温检测技术研究进展  

Advances in nucleic acid isothermal detection technologies for foodborne viruses

作　　者：秦智伟 薛亮[1] 高珺珊 胡永丹 张菊梅[1] 吴清平[1] QIN Zhiwei;XUE Liang;GAO Junshan;HU Yongdan;ZHANG Jumei;WU Qingping(Guangdong Institute of Microbiology,Guangdong Academic of Science,State Key Laboratory of Applied Microbiology in Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangzhou,Guangdong 510070,China;Institute of Agriculture and Food,Kunming University of Science and Technology,Kunming,Yunnan 650504,China)

机构地区：[1]广东省微生物研究所广东省科学院省部共建华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室,广东广州510070 [2]昆明理工大学农业与食品学院,云南昆明650504

出　　处：《微生物学通报》2021年第1期266-277,共12页Microbiology China

基　　金：国家重点研发计划(2017YFC1601200);广东省自然科学基金杰出青年基金(2019B151502065);广东省重点领域研发计划(2019B020209001);广东省科学院发展专项(2020GDASYL-20200104008)。

摘　　要：食源性病毒已成为全球引发食品安全事件的重要病原,对新型检测技术的不断发展提出了严峻的挑战。早期PCR技术在病原检测领域中的应用,推动了对食源性病毒的全面认识。近年来核酸恒温检测技术发展迅速,包括环介导等温扩增技术、重组酶聚合酶扩增技术、核酸序列依赖性扩增技术、链置换扩增技术、滚环扩增技术等,在抗复杂基质干扰、装备要求低以及可现场实时检测等方面具有明显的技术优势,已成为食源性病毒检测领域的热点研究方向。因此,本文对近年来食源性病毒核酸恒温检测技术的原理、应用、优缺点等方面进行综述,并对未来发展方向进行了展望。Foodborne viruses have become an important pathogen causing food safety incidents worldwide, which poses a severe challenge to the continuous development of new detection technologies. The application of PCR in the pathogen detection has promoted the comprehensive understanding of foodborne viruses. In recent years, nucleic acid thermostatic detection technologies have been developed rapidly, including loop-mediated isothermal amplification, recombinase polymerase amplification(RPA), nucleic acid sequence-based amplification(NASBA), strand displacement amplification, rolling circle amplification, etc. They showed obvious technical advantages in resisting complex matrix interference, low equipment requirements and on-site real-time detection, which become a hot direction in the foodborne virus detection. Therefore, this paper reviews the principles, applications, advantages and disadvantages of nucleic acid thermostatic detection technologies for foodborne viruses in recent years, and prospects the development direction in future.

关 键 词：食源性病毒 核酸 检测 环介导等温扩增 重组酶聚合酶扩增 核酸序列依赖性扩增 

分 类 号：R155.5[医药卫生—营养与食品卫生学] R440[医药卫生—公共卫生与预防医学]