食叶草粉对SD大鼠的致畸性研究  

Study on the teratogenicity of edible dock powder on SD rats

作　　者：楼敏涵 张丽婧 梅松 骆华星 刘臻 刘冬英 胡志航 陈建国 曲雪峰 胡文力 翟兵中 王茵 LOU Min-Han;ZHANG Li-Jing;MEI Song;LUO Hua-Xing;LIU Zhen;LIU Dong-Ying;HU Zhi-Hang;CHEN Jian-Guo;QU Xue-Feng;HU Wen-Li;ZHAI Bing-Zhong;WANG Yin(Health Food Research Institute,Hangzhou Medical College,Hangzhou 330013,China)

机构地区：[1]杭州医学院保健食品研究所,杭州330013

出　　处：《食品安全质量检测学报》2021年第3期945-950,共6页Journal of Food Safety and Quality

基　　金：浙江省医学支撑学科营养学项目(16-zc03);浙江省中医药科技计划重点项目(2019ZZ005);浙江省自然科学基金项目(LQ18H260003)。

摘　　要：目的探究食叶草粉对SD大鼠的母体毒性、胚胎毒性和致畸性。方法受孕SD大鼠按体重随机分组,每组不少于16只,设溶剂对照组(蒸馏水)和食叶草粉低、中、高剂量组(分别为0.42、0.84、1.67 g/kg.BW)。在受孕第6~15d,每日以10mL/kg.BW灌胃给予受试物或等量蒸馏水。观察、记录孕鼠临床表现和体重,于孕期第20 d称重并处死母鼠,检查孕鼠妊娠情况和胎鼠发育状况,包括黄体数、死胎数、吸收胎数、活胎数,胎仔性别、体重、体长、尾长,观察有无骨骼畸形和内脏畸形。结果将各剂量组的各项指标与溶剂对照组指标进行比较,差异无统计学意义(P>0.05)。结论食叶草粉在本实验条件下对SD大鼠无母体毒性、胚胎毒性和致畸性。Objective To explore the maternal toxicity,embryo toxicity and teratogenicity of edible dock powder in SD rats.Methods Pregnant SD rats were randomly divided into groups according to body weight,no less than 16 rats in each group.Solvent control group(distilled water)and low,medium and high dose groups of grass meal(0.42,0.84 and 1.67 g/kg.BW,respectively)were set.During the 6 th to the 15 th day of gestation,the maternal rats were orally given distilled water or edible dock power at 10 mL/kg.BW once daily,respectively.In the course of the study,clinical manifestations and body weights of the pregnant rats were observed and recorded weekly.The pregnant rats were weighted and humanely sacrificed on the 20 th day in pregnancy.Pregnancy of rats and fetal development were examined,including the numbers of corpus luteum,died fetuses,absorbed fetuses and living fetuses,the gender,body weight,body length,tail length and deformity of the fetal rats.Results There was no significant difference between the indexes of each dose group and those of the solvent control group(P>0.05).Conclusion Edible dock powder has no maternal toxicity,embryo toxicity and teratogenicity to SD rats under the experimental conditions。

关 键 词：食叶草 致畸性 胚胎 母体毒性 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]