Cryo-EM structure of the human mitochondrial translocase TIM22 complex  

作　　者：Liangbo Qi Qiang Wang Zeyuan Guan Yan Wu Cuicui Shen Sixing Hong Jianbo Cao Xing Zhang Chuangye Yan Ping Yin 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research,Huazhong Agricultural University,Wuhan,Hubei 430070,China [2]Public Laboratory of Electron Microscopy,Huazhong Agricultural University,Wuhan,Hubei 430070,China [3]Department of Biophysics,and Department of Pathology of Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Center of Cryo Electron Microscopy,Zhejiang University,Hangzhou,Zhejiang 310058,China [4]Beijing Advanced Innovation Center for Structural Biology,Tsinghua-Peking Joint Center for Life Sciences,School of Life Sciences,Tsinghua University,Beijing 100084,China

出　　处：《Cell Research》2021年第3期369-372,共4页细胞研究（英文版）

基　　金：This work was supported by funds from the Ministry of Science and Technology of China(2018YFA0507700);the National Natural Science Foundation of China(31722017);the Fok Ying-Tong Education Foundation(151021);the Fundamental Research Funds for the Central Universities(2662017PY031);This research was supported by Beijing Advanced Innovation Center for Structural Biology(to Dr.Chuangye Yan).

摘　　要：Dear Editor,Mitochondria are essential organelles in cellular metabolism,homeostasis,and apoptosis.1,2 Most mitochondrial proteins are synthesized as precursors in the cytosol and then imported into mitochondria by specific protein translocase complexes,including the translocase of the outer membrane complex(TOM complex),the carrier translocase of the inner membrane complex(TIM22 complex),the presequence translocase of the inner membrane complex(TIM23 complex),the sorting and assembly machinery(SAM complex),and the mitochondrial import complex(MIM complex).3 The TIM22 complex is responsible for the translocation and insertion of hydrophobic membrane proteins,including mitochondrial carrier proteins and translocase subunits(Tim17,Tim22 and Tim23).3 In humans,TIM22 is a 440-kDa complex comprising at least six components:the hypothetical channel-forming protein Tim22,three small Tim proteins(Tim9,Tim10a and Tim10b),Tim29 and acylglycerol kinase(AGK).1 Considering the functional importance of mitochondrial protein import,the TIM22 complex has been linked to many diseases.For example,mutations in the TIM22 gene have been reported to cause early-onset mitochondrial myopathy.4 AGK participates in lipid biosynthesis,and mutations in the AGK gene lead to Sengers syndrome.2 Mutations in the TIMM8A gene(also called DDP1)cause deafness dystonia syndrome.

关 键 词：TRANS INNER COMPLEXES 

分 类 号：Q2[生物学—细胞生物学]