结直肠癌组织中lncRNA FOXC2-AS1与EZH2的表达及临床意义  

Expression and clinical significance of lncRNA FOXC2-AS1 and EZH2 in colorectal cancer tissues

作　　者：赵钢艳 熊将军[1] 刘灵芝 王明明 王璨 易小明 ZHAO Gangyan;XIONG Jiangjun;LIU Lingzhi;WANG Mingming;WANG Can;YI Xiaoming(Department of Clinical Laboratory,the First Affiliated Hospital of Hunan College of Traditional Chinese Medicine/Hunan Provincial Hospital of Traditional Chinese Medicine,Zhuzhou,Hunan 412000,China)

机构地区：[1]湖南中医药高等专科学校附属第一医院/湖南省直中医院检验科,湖南株洲412000

出　　处：《国际检验医学杂志》2021年第7期819-823,共5页International Journal of Laboratory Medicine

基　　金：湖南省卫生健康委员会科研课题(C2019141)。

摘　　要：目的探讨结直肠癌(CRC)组织中长链非编码RNA(lncRNA)RNA FOXC2-AS1、Zeste基因增强子同源物2(EZH2)表达及临床意义。方法采用实时荧光定量PCR检测86例CRC癌组织和癌旁组织中lncRNA FOXC2-AS1、EZH2的表达。采用免疫组化法检测癌组织和癌旁组织中EZH2的蛋白表达。采用Pearson相关分析lncRNA FOXC2-AS1与EZH2表达的相关性。统计学分析癌组织中lncRNA FOXC2-AS1、EZH2的表达与临床病理特征之间的关系。Kaplan-Meier生存曲线分析不同lncRNA FOXC2-AS1、EZH2表达水平患者的5年总体生存率的差异。结果与癌旁组织比较,癌组织中lncRNA FOXC2-AS1及EZH2 mRNA表达水平升高(P<0.05),EZH2蛋白表达阳性率较高(P<0.05)。癌组织中lncRNA FOXC2-AS1与EZH2 mRNA表达水平呈正相关(r=0.623,P<0.05)。癌组织中lncRNA FOXC2-AS1及EZH2表达与肿瘤分期有关(P<0.05),与患者的性别、年龄、肿瘤大小、肿瘤位置、肿瘤分化程度及是否伴淋巴结转移无关(P>0.05)。Kaplan-Meier生存曲线分析结果显示lncRNA FOXC2-AS1及EZH2高表达的患者5年总体生存率低于lncRNA FOXC2-AS1及EZH2低表达患者(P<0.05)。结论CRC癌组织中lncRNA FOXC2-AS1及EZH2表达水平升高,lncRNA FOXC2-AS1及EZH2的高表达与肿瘤分期有关,二者有望成为新的评价肿瘤预后的分子标志物。Objective To investigate the expression and clinical significance of long non-coding RNA(lncRNA)FOXC2-AS1 and Zeste gene enhancer homolog 2(EZH2)in colorectal cancer(CRC)cancer tissue.Methods Real-time fluorescent quantitative PCR was used to detect the expression of lncRNA FOXC2-AS1 and EZH2 in 86 cases of CRC cancer tissues and adjacent tissues.Immunohistochemistry was used to detect the protein expression of EZH2 in cancerous tissues and adjacent tissues.Pearson linear correlation analysis lncRNA FOXC2-AS1 and EZH2 expression correlation.The relationship between the expression of lncRNA FOXC2-AS1,EZH2 and clinicopathological characteristics in cancer tissues was analyzed by statistics.Kaplan-Meier survival curve was used to analyze the difference of 5-year overall survival rate of patients with different lncRNA FOXC2-AS1 and EZH2 expression.Results Compared with adjacent tissues,the expression levels of lncRNA FOXC2-AS1 and EZH2 mRNA in cancer tissues were increased(P<0.05),and the positive rate of EZH2 protein expression was higher(P<0.05).There was a positive correlation between lncRNA FOXC2-AS1 and EZH2 mRNA expression levels in cancer tissues(r=0.623,P<0.05).The expression of lncRNA FOXC2-AS1 and EZH2 in cancer tissues were related to tumor stage(P<0.05),but not related to the patient′s gender,age,tumor size,tumor location,tumor differentiation,and lymph node metastasis(P>0.05).Kaplan-Meier survival curve analysis showed that the 5-year overall survival rate of patients with high lncRNA FOXC2-AS1 and EZH2 expression were lower than that of patients with low lncRNA FOXC2-AS1 and EZH2 expression(P<0.05).Conclusion The expressions of lncRNA FOXC2-AS1 and EZH2 are up-regulated in CRC cancer tissues.The high expressions of lncRNA FOXC2-AS1 and EZH2 are related to tumor stage,and they are expected to become new molecular markers for evaluating tumor prognosis.

关 键 词：结直肠癌 长链非编码RNA FOXC2-AS1 Zeste基因增强子同源物2 

分 类 号：R735.35[医药卫生—肿瘤]