人类白细胞抗原DR的表达水平与HBV感染不同转归的关系  

The relationship between the expression level of human leukocyte antigen DR and the different outcomes of HBV infection

作　　者：樊小群 李欢[1] 朱华雄 蔡庆果 何吕芬 FAN Xiaoqun;LI Huan;ZHU Huaxiong;CAI Qingguo;HE Lyufen(Department of Clinical Laboratory,Sanya People′s Hospital,Sanya,Hainan 572000,China)

机构地区：[1]海南省三亚市人民医院检验科,海南三亚572000

出　　处：《国际检验医学杂志》2021年第7期852-855,共4页International Journal of Laboratory Medicine

基　　金：海南省自然科学基金青年基金项目(818QN326)。

摘　　要：目的探讨人类白细胞抗原(HLA)-DR mRNA表达水平与HBV感染不同转归的关系。方法收集该院2014年8月至2017年10月住院及门诊确诊的乙型肝炎病毒(HBV)急性感染者7例、急性自限性者30例和慢性感染者64例(乙型肝炎病毒轻度感染24例,中度感染12例,重度感染8例,携带者20例)和体检健康者53例(疫苗免疫者35例和HBV 5项全阴者18例)的临床资料。采用实时荧光定量PCR检测各组HBV-DNA水平和PBMC中HLA-DR mRNA表达水平。结果HLA-DR mRNA表达水平在急性、急性自限、慢性、疫苗免疫和5项全阴组中比较,急性自限组最高,5项全阴组最低,组间比较差异有统计学意义(F=9.75,P<0.05)。其中,急性自限组与慢性组HLA-DR mRNA表达水平高于疫苗免疫组(t=4.767、4.576,P<0.05);慢性组、疫苗免疫组HLA-DR mRNA表达水平均高于5项全阴组(t=7.346、3.374,均P<0.05)。不同HBV-DNA水平组中HLA-DR mRNA表达水平比较,差异无统计学意义(F=1.204,P=0.319);不同程度慢性HBV感染组中,HLA-DR mRNA表达水平在重度组中最高,但组间比较差异无统计学意义(F=0.540,P=0.657)。结论不同疾病阶段的HBV感染和疫苗免疫均可诱导HLA-DR mRNA表达水平升高,在急性自限状态下最为明显;慢性HBV感染中HLA-DR mRNA表达水平可能与细胞损伤程度有关。Objective To investigate the relationship between the expression of human leukocyte antigen(HLA)-DR mRNA and the different outcomes of HBV infection.Methods From August 2014 to October 2017,7 cases of acute hepatitis B virus(HBV)infection,30 cases of acute self-limiting,64 cases of chronic infection(24 cases of mild infection of hepatitis B virus,12 cases of moderate infection,8 cases of severe infection,20 cases of carriers),and 53 cases of healthy people(35 cases of vaccine-immunized patients and 18 cases of HBV 5 negative cases)who were hospitalized and diagnosed in the outpatient department of the hospital were collected.Real-time fluorescence quantitative PCR was used to detect the levels of HBV-DNA and HLA-DR mRNA expression in PBMC in each group.Results Among the acute,acute self-limiting,chronic,vaccine-immunized and 5-item negative patients,the HLA-DR mRNA expression level was the highest in the acute self-limiting group,and the 5-item negative group was the lowest.The difference between the groups were statistically significant(F=9.75,P<0.05).Among them,the HLA-DR mRNA expression levels of the acute self-limiting group and the chronic group were higher than that of the vaccine immunization group(t=4.767,4.576,P<0.05);the HLA-DR mRNA expression levels of the chronic group and the vaccine immunization group were higher than that of 5-item group(t=7.346,3.374,P<0.05).There were no statistically significant differences in HLA-DR expression levels in different HBV-DNA level groups(F=1.204,P=0.319).In different degrees of chronic HBV infection groups,the HLA-DR expression levels was the highest in the severe group,but the differences between the groups were not statistically significant(F=0.540,P=0.657).Conclusion HBV infection and vaccine immunization at different disease stages can induce the increase of HLA-DR expression level,which is most obvious in the acute self-limiting state;HLA-DR expression level in chronic HBV infection may be related to the degree of cell damage.

关 键 词：人类白细胞抗原-DR 乙型肝炎病毒 不同转归 

分 类 号：R575.1[医药卫生—消化系统]