重组人促红细胞生成素对创伤性脑损伤大鼠炎性因子及线粒体损伤的影响  

Effect of recombinant human erythropoietin on inflammatory factors and mitochondrial damage in brain tissue of rats with traumatic brain injury

作　　者：詹亚琨[1] 罗艳[2] 邹蓓 ZHAN Yakun;LUO Yan;ZOU Bei(Emergency Department of the Second Affiliated Hospital of Nanchang University,Nanchang 330006,China;Image Center of the Second Affiliated Hospital of Nanchang University,Nanchang 330006;Laboratory Department of the Second Affiliated Hospital of Nanchang University,Nanchang 330006)

机构地区：[1]南昌大学第二附属医院急诊科,南昌330006 [2]南昌大学第二附属医院影像中心,南昌330006 [3]南昌大学第二附属医院检验科,南昌330006

出　　处：《中国比较医学杂志》2021年第3期96-103,共8页Chinese Journal of Comparative Medicine

摘　　要：目的研究重组人促红细胞生成素(recombinant human erythropoietin,rh EPO)对创伤性脑损伤大鼠炎性因子及线粒体损伤的影响。方法将SPF级SD雄性大鼠随机分为三组,每组15只,分别为假手术组(sham组),模型组、rh EPO干预组(治疗组),模型组、治疗组采用改良过的Feeney自由落体撞击头部来建立创伤性大鼠模型,sham组大鼠不撞击脑部。造模结束后,治疗组每日定时以5000 IU/kg剂量rh EPO进行腹腔注射,sham组和模型组腹腔注射等剂量生理盐水。连续给药7 d后处死大鼠。罗丹明123(rhodamine 123,Rh 123)染色检测脑组织线粒体膜电位改变。免疫组化检测脑组织中白细胞介素-1β(interleukin-1β,L-1β),白细胞介素6(interleukin-6,IL-6)和肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)表达。蛋白免疫印迹检测脑组织中线粒体动力学有关蛋白(动力相关蛋白1(Drp-1)、分裂蛋白1(Fis-1)、融合蛋白2(Mfn 2)、视神经萎缩蛋白1 (Opa 1)的表达水平。结果与sham组相比,模型组脑组织Rh123荧光强度明显减弱,IL-1β、IL-6、TNF-α、Drp-1、Fis-1蛋白的表达明显升高,Mfn 2、Opa 1蛋白的表达明显降低(均P<0.05)。与模型组相比,治疗组脑组织Rh123荧光强度明显增强,IL-1β、IL-6、TNF-α、Drp-1、Fis-1蛋白的表达明显降低,Mfn 2、Opa 1蛋白的表达明显升高(均P<0.05)。结论重组人促红细胞生成素可以减轻创伤性脑损伤后的炎性反应及线粒体损伤,从而起到对创伤性脑损伤后脑组织的保护作用。Objective We investigated the effects of recombinant human erythropoietin(rh EPO)on inflammatory factors and mitochondrial damage in the brain tissue of rats with traumatic brain injury.Methods Sprague-Dawley rats were randomly divided into three groups,15 in each group:a sham operation group(sham group),a model group,and a rh EPO intervention group(treatment group).The rats in the model group and the treatment group were subjected to a modified Feeney weight-drop model,in which the head strike establishes the traumatic rat model;the sham group was not subjected to this head strike.At the end of modeling,the treatment group was intraperitoneally injected with rh EPO at a dose of 5000 IU/kg daily.The sham group and the model group were intraperitoneally injected with an equal amount of normal saline.The rats were sacrificed seven days after continuous administration.rhodamine 123(Rh123)staining was used to detect changes in mitochondrial membrane potential.Immunohistochemistry was used to detect the expression of interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in the brain tissue of each group.Western blot analysis was used to detect the protein expression levels of dynamin-related protein 1(Drp1),mitochondrial fission 1 protein(Fis1),mitochondrial fusion protein 2(Mfn2),and optic atrophic protein 1(Opa1).Results Compared with the sham group,the Rh123 fluorescence intensity of brain tissue cells in the model group was significantly weakened,the expressions of IL-1β,IL-6,TNF-α,Drp1,and Fis1 proteins were significantly increased,and the expressions of MFN2 and OPA1 proteins were significantly decreased(all P<0.05).Compared with the model group,the Rh123 fluorescence intensity of brain tissue cells in the treatment group was significantly increased,the expressions of IL-1β,IL-6,TNF-α,Drp1,Fis1 proteins were significantly decreased,and the expressions of Mfn2 and OPA1 proteins were significantly increased(all P<0.05).Conclusions Recombinant human erythropoietin can reduce inflammato

关 键 词：重组人促红细胞生成素 创伤性脑损伤 炎性反应 线粒体损伤 

分 类 号：R-33[医药卫生]