血清C-反应蛋白和内皮素水平检测与急性胆囊炎临床分级的相关性分析  

Correlation Analysis of Serum C-reactive Protein and Endothelin Levels with Clinical Grade of Acute Cholecystitis

作　　者：高伟[1] 席锐[1] 李涛[1] GAO Wei;XI Rui;LI Tao(Department of Hepatobiliary Surgery,Hanzhong Central Hospital,Shaanxi Hanzhong 723000,China)

机构地区：[1]汉中市中心医院肝胆外科,陕西汉中723000

出　　处：《现代检验医学杂志》2021年第2期144-147,共4页Journal of Modern Laboratory Medicine

摘　　要：目的探究血清C-反应蛋白(C-reactive protein,CRP)、内皮素(endothelin,ET)水平检测与急性胆囊炎(acute cholecystitis,AC)临床分级的相关性。方法选取汉中市中心医院2017年3月~2019年11月收治的110例AC患者为观察组,另选取同期110例健康体检者为对照组,分别收集两组的吸烟、饮酒、高血压、CRP和ET水平等一般资料。采用酶联免疫吸附测定法(ELISA)检测人血清中CRP水平,采用放射免疫法(radioimmunoassay,RIA)检测人血清中ET水平。采用Spearson相关性检验分析CRP,ET联合与AC临床分级的相关性。结果两组的性别、平均年龄、吸烟、饮酒、高血压、糖尿病差异均无统计学意义(χ^(2)/t=1.640,0.428,3.160,4.250,3.570,3.660,均P>0.05)。两组的CRP和ET水平差异具有统计学意义(t=9.629,7.374,均P<0.05),且观察组患者的CRP和ET水平显著高于对照组。ROC曲线分析CRP,ET和二者联合诊断AC的曲线下面积(AUC)分别是0.843(95%CI:0.771~0.916,P=0.000),0.772(95%CI:0.683~0.860,P=0.000)和0.910(95%CI:0.851~0.969,P=0.000),约登指数最大值对应的CRP,ET为诊断AC的最佳截距值(cut-off)分别是8.2 mg/L和72.5 pg/ml,CRP与ET联合诊断AC的灵敏度,特异度均高于单独使用CRP和ET。CRP,ET联合与AC分级呈正相关关系(r=0.386,P<0.05)。结论CRP和ET联合水平可区分不同严重程度的AC。CRP,ET联合与AC分级呈正相关关系,值得深入研究和应用。Objective To explore the correlation between serum C-reactive protein(CRP)and endothelin(ET)levels and the clinical grade of acute cholecystitis(AC).Methods A total of 110 AC patients admitted to Hanzhong Central Hospital from March 2017 to November 2019 were selected as the observation group,and 110 healthy physical check-up patients in the same period were selected as the control group.General data on smoking,alcohol consumption,hypertension,CRP and ET levels in the two groups were collected respectively.The levels of CRP and ET in human serum were measured by ELISA and radioimmunoassay(RIA).Spearson correlation test was used to analyze the correlation between CRP,ET and AC clinical grading.Results There were no statistically significant differences in gender,average age,smoking,alcohol consumption,hypertension or diabetes between the two groups((χ^(2)/t=1.640,0.428,3.160,4.250,3.570,3.660,all P>0.05).The difference of CRP and ET levels between the two groups was statistically significant(t=9.629,7.374,all P<0.05),and the CRP and ET levels of patients in the observation group were significantly higher than those in the control group.In ROC curve analysis,the AUC of CRP,ET and the combined diagnosis of AC was 0.843(95%CI:0.771~0.916,P=0.000),0.772(95%CI:0.683~0.860,P=0.000)and 0.910(95%CI:0.851~0.969,P=0.000),respectively,the maximum value of the Jordan index corresponding to CRP and ET were 8.2 mg/L and 72.5 pg/ml,respectively,and the sensitivity and specificity of CRP and ET in the combined diagnosis of AC were higher than that of CRP and ET alone.CRP and ET were positively correlated with AC grading(r=0.386,P<0.05).Conclusion The combined levels of CRP and ET can distinguish AC with different degrees of severity,and the combined levels of CRP and ET are positively correlated with AC grading,which is worthy of further study and application.

关 键 词：急性胆囊炎 C-反应蛋白 内皮素 

分 类 号：R576[医药卫生—消化系统] R446.11[医药卫生—内科学]