BFAR在胶质瘤中的表达及其与胶质瘤预后的关系  

The Expression of BFAR in Glioma and Its Relationship with the Prognosis of Glioma

作　　者：程哲 汪潮潮 吴娟[1] 王棒 周星辰 CHENG Zhe;WANG Chao-chao;WU Juan;WANG Bang;ZHOU Xing-chen(Department of Neurosurgery,Second Affiliated Hospital of Bengbu Medical College,Bengbu 233000,Anhui,China)

机构地区：[1]蚌埠医学院第二附属医院神经外科,安徽蚌埠233000

出　　处：《医学信息》2021年第7期78-81,共4页Journal of Medical Information

基　　金：安徽自然科学基金项目(编号:1908085QH334);蚌埠医学院研究生科研创新计划项目(编号:Byycx20033)。

摘　　要：目的探讨双功能凋亡调节器(BFAR)在胶质瘤中的表达情况及其与胶质瘤患者预后的关系。方法基于美国癌症基因数据库(TCGA)及中国脑胶质瘤基因谱数据库(CGGA),分析胶质瘤临床标本中BFAR的表达情况及其与胶质瘤患者预后之间的关系、与凋亡相关基因的表达关系;通过Western blot及qRT-PCR验证BFAR蛋白及mRNA在胶质瘤细胞系中的表达情况。结果基于TCGA数据库分析显示,低级别胶质瘤BFAR的表达高于非瘤脑组织(P<0.05);胶质母细胞瘤BFAR表达高于非瘤脑组织(P<0.05),且随着胶质瘤恶性程度增加,BFAR的表达量增加。BFAR低表达的胶质瘤患者总体生存时间长于BFAR高表达的胶质瘤患者(P<0.05);高表达BFAR的高级别胶质瘤患者总体生存期短于低表达BFAR的高级别胶质瘤患者(P<0.05);异柠檬酸脱氢酶(IDH)野生型胶质瘤患者BFAR表达高于IDH突变型患者(P<0.05);非1p/19q联合缺失的胶质瘤患者BFAR表达高于1p/19q联合缺失的患者(P<0.05)。基于TCGA数据库分析显示,胶质瘤中BFAR与抗凋亡相关蛋白Bcl-xl、Bcl-w、Mcl-1表达及促凋亡相关蛋白Bax、Bad、Bim表达密切相关(P<0.05)。BFAR mRNA在胶质瘤细胞U251、T98和LN229中的表达比正常星形胶质细胞NHA中高(P<0.05)。结论BFAR在胶质瘤组织中及细胞系中高表达,其与胶质瘤患者的预后密切相关。Objective To investigate the expression of dual function apoptosis regulator(BFAR)in glioma and its relationship with the prognosis of patients with glioma.Methods Based on The Cancer Genome Altas(TCGA)and the Chinese Glioma Genome Altas(CGGA),analyze the expression of BFAR in clinical specimens of glioma and its relationship with the prognosis of patients with glioma,and the relationship with the expression of apoptosis-related genes;The expression of BFAR protein and mRNA in glioma cell lines was verified by Western blot and qRT-PCR.Results Based on the analysis of the TCGA database,the expression of BFAR in low-grade gliomas was higher than that of non-tumor brain tissues(P<0.05);The expression of BFAR in glioblastoma was higher than that in non-tumor brain tissue(P<0.05),and as the malignant degree of glioma increases,the expression of BFAR increases.The overall survival time of glioma patients with low expression of BFAR was longer than that of patients with high expression of BFAR(P<0.05);The overall survival time of patients with high-grade glioma with high expression of BFAR was shorter than that of patients with high-grade glioma with low expression of BFAR(P<0.05);Isocitrate dehydrogenase(IDH)wild-type glioma patients had higher BFAR expression than IDH mutant patients(P<0.05);The expression of BFAR in glioma patients without 1p/19q combined deletion was higher than that in patients with 1p/19q combined deletion(P<0.05).Based on the analysis of the TCGA database,BFAR is closely related to the expression of anti-apoptosis-related proteins Bcl-xl,Bcl-w,and Mcl-1 and the expression of pro-apoptosis-related proteins Bax,Bad,and Bim in gliomas(P<0.05).The expression of BFAR mRNA in glioma cells U251,T98 and LN229 was higher than that in normal astrocytes NHA(P<0.05).Conclusion BFAR is highly expressed in glioma tissues and cell lines,and it is closely related to the prognosis of glioma patients.

关 键 词：BFAR 胶质瘤 MRNA 

分 类 号：R739.41[医药卫生—肿瘤]