Profiling of miRNAs in porcine Sertoli cells  

作　　者：Xiaoxu Chen Yi Zheng Xueliang Li Qiang Gao Tongying Feng Pengfei Zhang Mingzhi Liao Xiu’e Tian Hongzhao Lu Wenxian Zeng 

机构地区：[1]College of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723001,China [2]Key Laboratory of Animal Genetics,Breeding and Reproduction of the Ministry of Agriculture,College of Animal Science and Technology,Northwest A&F University,Yangling 712100,Shaanxi,China [3]College of Life Science,Northwest A&F University,Yangling 712100,Shaanxi,China.

出　　处：《Journal of Animal Science and Biotechnology》2021年第1期236-249,共14页畜牧与生物技术杂志（英文版）

基　　金：supported in part by the National Natural Science Foundation of China (Grant No. 31572401, 31772605) to W.Z。

摘　　要：Background: Sertoli cells(SCs) create a specialized environment to support and dictate spermatogenesis.MicroRNAs(miRNAs), a kind of ~ 22 nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely unknown.Methods: We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of porcine SCs. We screened the highly enriched SC miRNAs and predicted their functions by Gene Ontology analysis. The dual luciferase assay was used to elucidate the regulation of tumor necrosis factor receptor(TNFR)-associated factor 3(TRAF3) by ssc-miR-149.Results: The analysis showed that 18 miRNAs were highly expressed in SCs and 15 miRNAs were highly expressed in germ cells. These miRNAs were predicted to mediate SC and germ cell functions. In addition, ssc-miR-149 played critical roles in SCs by targeting TRAF3.Conclusion: Our findings provide novel insights into the miRNA expression pattern and their regulatory roles of porcine SCs.

关 键 词：MICRORNAS PIG Sertoli cell SPERMATOGENESIS TRAF3 

分 类 号：S828.2[农业科学—畜牧学]