Edible Bird's Nest Attenuates Menopause-Related Bone Degeneration in Rats via Increasing Bone Estrogen-Receptor Expression  

作　　者：HOU Zhi-ping TANG Shi-ying Jl Hai-ru HE Pei-yuan LI Yu-hong DONG Xian-ling DU Meng-nan Ismail Maznah HE Wen-jing 

机构地区：[1]Department of Pathology,Chengde Medical University,Chengde,Hebei Province(067000),China [2]Department of Gastroenterology,The Affiliated Hospital of Chengde Medical University,Chengde,Hebei Province(067000),China [3]Department of Pathology The Affiliated Hospital of Hebei University,Baoding,Hebei Province(0671000),China [4]Laboratory of Molecular Biomedicine,Institute of Bioscience,Universiti Putra Malaysia,Serdang,Selangor(43400),Malaysia [5]Department of Gastroscopy,Kuancheng Manchu Autonomous County Hospital,Chengde,Hebei Province(067000),China

出　　处：《Chinese Journal of Integrative Medicine》2021年第4期280-285,共6页中国结合医学杂志（英文版）

基　　金：Supported by Grants from Ministry of Science,Technology and Innovation,E-science Fund(No.5450666);Malaysia;the Health and Family Planning Commission of Hebei(No.20160315);the Administration of Traditional Chinese Medicine of Hebei(No.2017196);the Key Discipline Construction Project of Hebei Provincial Universities[No.JiJiao Gao-2013-(4)-2012-37];Start up Fund for High Level Talents of Chengde Medical University(No.201704)。

摘　　要：Objective:To investigate the mechanistic basis for the attenuation of bone degeneration by edible bird's nest(EBN)in ovariectomized rats.Methods:Forty-two female Sprage-Dawley rats were randomized into 7 groups(6 in each group).The ovariectomized(OVX)and OVX+6%,3%,and 1.5%EBN and OVX+estrogen groups were given standard rat chow alone,standard rat chow+6%,3%,and 1.5%EBN,or standard rat chow+estrogen therapy(0.2mg/kg per day),respectively.The sham-operation group was surgically opened without removing the ovaries.The control group did not have any surgical intervention.After 12 weeks of intervention,blood samples were taken for serum estrogen,osteocalcin,and osteoprotegerin,as well as the measurement of magnesium,calcium abd zinc concentrations.While femurs were removed from the surrounding muscles to measure bone mass density using the X-ray edge detection technique,then collected for histology and estrogen receptor(ER)immunohistochemistry.Results:Ovariectomy altered serum estrogen levels resulting in increased food intake and weight gain,while estrogen and EBN supplementation attenuated these changes.Ovariectomy also reduced bone ER expression and density,and the production of osteopcalcin and osteorotegerin,which are important pro-osteoplastic hormones that promote bone mineraliztion and density.Conversely,estrogen and EBN increased serum estrogen levels leading to increased bone ER expression,pro-osteoplastic hormone production and bone density(all P<0.05).Conclusion:EBN could be used as a safe alternative to hormone replacement therapys for managing menopausal complications like bone degeneration.

关 键 词：edible bird's nest MENOPAUSE OVARIECTOMY RATS BONE osteoporosis 

分 类 号：R285.5[医药卫生—中药学]