Stanford A型主动脉夹层血管壁及外周血中热休克蛋白90的表达及意义  

Expression and Significance of Heat Shock Protein 90 in the Aortic Wall and Peripheral Blood of Stanford Type A Aortic Dissection

作　　者：袁瑞军 赵志敏 唐杨烽 王国坤 韩林 徐志云 YUAN Rui-jun;ZHAO Zhi-min;TANG Yang-feng;WANG Guo-kun;HAN Lin;XU Zhi-yun(Department of Cardiovascular Surgery,The First Affiliated Hospital of Naval Medical University,Shanghai,200433,China)

机构地区：[1]海军军医大学第一附属医院心血管外科,上海200433

出　　处：《现代生物医学进展》2021年第1期8-12,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81870344)。

摘　　要：目的:探索热休克蛋白90(Heat shock protein 90, HSP90)在Stanford A型主动脉夹层(Aortic dissection, AD)血管壁组织中的表达及其与平滑肌细胞(Smooth muscle cells, SMCs)表型标志物的相关性。方法:收集本院急性Stanford A型主动脉夹层患者病变主动脉壁组织和外周血标本(AD组,20例),心脏移植供体等来源的正常主动脉壁组织和外周血标本(正常组,10例);ELISA法检测血浆HSP90含量;免疫组化染色检测HSP90的表达差异及定位;Western blot检测组织标本中蛋白的表达差异;应用Spearman分析HSP90和SMCs表型标志物表达的相关性。结果:AD患者血浆中HSP90的含量显著高于正常组(142.38±40.16ng/mL vs. 54.99±24.46 ng/mL,P<0.01)。相对于正常主动脉壁组织,主动脉夹层血管壁组织中HSP90表达明显升高,且主要分布在血管壁中层细胞的胞浆中,分泌型SMCs标志物OPN的表达明显增多,而收缩型标志物α-SMA表达则显著减少。HSP90和α-SMA的蛋白表达呈负相关(R2=0.677,P<0.01),和OPN呈正相关(R^(2)=0.572,P<0.01)。结论:主动脉夹层患者的血管壁组织及外周血中的HSP90含量明显升高,参与了主动脉夹层的发生发展。Objective: To investigate the expression of heat shock protein 90(HSP90) in the aortic wall of Stanford type A aortic dissection(AD) and its relationship with the expressions of phenotype markers of smooth muscle cells(SMCs). Methods: The aortic wall tissue and peripheral blood samples from patients with Stanford Type A aortic dissection(AD group, 20) and donors for heart transplant(normal group, 10) were collected. Plasma HSP90 was detected by Elisa;Immunohistochemical staining was used to detect the difference of HSP90 expression in paraffin sections of the above tissue specimens. The protein expression levels were detected by Western blot.Spearman analysis was used to analyze the correlation between HSP90 expression and SMCs phenotype makers. Results: The plasma level of HSP90 in patients with AD was significantly higher than that in the normal group(142.38±40.16 ng/mL vs 54.99±24.46 ng/mL,P<0.01). Compared with normal aortic walls, the protein expression level of HSP90 in the aortic wall tissue of AD was significantly increased and mainly concentrated in the cytoplasm of SMCs in the medial wall. OPN, marker of synthetic SMCs, was also increased,whereas α-SMA, marker of contractile SMCs, was significantly decreased. There was a negative correlation between the protein expressions of HSP90 and α-SMA(R2=0.677, P<0.01), and positive correlation between HSP90 and OPN(R2=0.572, P<0.01).Conclusions: The expression of HSP90 in the aortic wall of AD and the level of HSP90 in the patient’s blood were significantly increased,and was involved in the occurrence and development of AD.

关 键 词：热休克蛋白90 表型转化 平滑肌细胞 主动脉夹层 

分 类 号：R-33[医药卫生] R543.1