亚麻籽蛋白质、亚麻酸和木酚素含量近红外预测模型建立与应用  

Establishment and applicant of near-infrared reflectance spectroscopy models for predicting protein,linolenic acid and lignan contents of flaxseed

作　　者：叶佳丽 江海霞[1] 郭栋良 闫文亮 杨亮杰 黄丽丽 陈大伟 李恭泽 谢丽琼[1] YE Jia-li;JIA Hai-xia;GUO Dong-liang;YAN Wen-liang;YANG Liang-jie;HUANG Li-li;CHEN Da-wei;LI Gong-ze;XIE Li-qiong(College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)

机构地区：[1]新疆大学生物科学与技术学院,新疆乌鲁木齐830046

出　　处：《中国油料作物学报》2021年第2期353-360,共8页Chinese Journal of Oil Crop Sciences

基　　金：新疆自治区科技厅项目(PT1808)。

摘　　要：为优化油用亚麻营养品质检测方法,提高育种效率,利用无损近红外光谱分析法定量分析亚麻籽粒蛋白质、亚麻酸和木酚素含量,初步探讨蛋白质、亚麻酸和木酚素含量相关性。以具有代表性的200份亚麻籽为研究对象,采集近红外光谱,使用偏最小二乘法和净分析信号光谱预处理建立了蛋白质、亚麻酸和木酚素含量近红外模型。结果表明:亚麻籽蛋白质、亚麻酸和木酚素含量建模标准偏差分别为0.7526、0.5943和0.1483;建模相关系数分别为0.9784、0.9969和0.9943;预测平均偏差分别为-0.2441、1.2271和0.0521;预测标准偏差分别为2.7896、8.2459和1.0163;外部检验相关系数分别为0.9207、0.8885和0.9659;200份亚麻种质蛋白质、亚麻酸和木酚素含量符合正态性检验;相同环境木酚素与亚麻酸含量均呈现正相关、木酚素与蛋白质含量均呈现负相关、亚麻酸与蛋白质含量呈现负相关。使用模型,从863份亚麻种质资源库中筛选出亚麻酸70%以上种质12份,蛋白质含量低于10%以下种质8个,木酚素含量高于10%种质11份。本研究将为亚麻重要品质性状改良提供技术和材料。Near infrared spectrum models were established using 200 flaxseed(Linum usitatissimum L.) germplasm. Contents of protein, ALA(linolenic acid) and SDG(lignan) were sampled by partial least square method(PLS) and net analysis signal algorithm(NAS) spectral preprocessing. Results showed that the standard errors of calibration for contents of protein, ALA and SDG were 0.7526, 0.5943 and 0.1483. Their modelling correlation coefficients were 0.9784, 0.9969 and 0.9943. The predicted average deviations were-0.2441, 1.2271 and 0.0521;predicted standard deviations were 2.7896, 8.2459 and 1.0163. The correlation coefficients of external tests were0.9207, 0.8885 and 0.9659. The contents of protein, ALA and SDG of 200 flaxseed samples conformed to the normality test. In the same environment, contents of SDG and ALA were positively correlated, the contents of SDG and protein were negatively correlated, and the contents of ALA and protein were negatively correlated. Among 863 gemplasm materials, 12 of them had high ALA(more than 70%) and 8 had low protein(less than 10%), and 11 had high SDG(more than 10%).This study provided valuable tools and materials for the genetic improvement of flaxseed.

关 键 词：亚麻籽 蛋白质 亚麻酸 木酚素 近红外模型 

分 类 号：S565.9[农业科学—作物学]