基于Bax/Bcl-2-Caspase-3通路芍药软肝方抑制裸鼠肝癌生长及机制研究  

Study of the inhibitory effect of Shaoyao Ruangan Formula on the growth of liver cancer in nude mice based on Bax/Bcl-2-Caspase-3 pathway and its mechanism

作　　者：李骏飞 郑小蓉 姜建伟 章红燕 LI Junfei;ZHENG Xiaorong;JIANG Jianwei;ZHANG Hongyan(The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital),Institute of Basic Medicine and Cancer(IBMC),Chinese Academy of Sciences,Hangzhou310022,China)

机构地区：[1]中国科学院大学附属肿瘤医院(浙江省肿瘤医院)中国科学院基础医学与肿瘤研究所,浙江杭州310022

出　　处：《中国现代医生》2021年第7期38-41,45,F0003,共6页China Modern Doctor

基　　金：浙江省中医药科技计划项目(2016ZB020)。

摘　　要：目的研究芍药软肝方(SRF)调控Bax/Bcl-2-Caspase-3通路抑制肝癌生长的作用及机制。方法建立HepG2荷瘤裸鼠模型,随机分为空白对照组、高、中、低浓度芍药软肝方(SRF)组,灌胃3周后称取瘤重,TUNEL法观察肿瘤细胞凋亡情况,Western-blot法检测对组织中NF-κB-p65、VEGF、Bcl-2、Bax、Caspase-3蛋白质表达。结果(1)不同浓度芍药软肝方(SRF)均可抑制HepG2荷瘤裸鼠体内肝癌肿瘤的生长,并能诱导肿瘤细胞凋亡;(2)SRF可降低裸鼠肝癌组织中NF-κB-p65、VEGF、Bcl-2蛋白表达,上调Bax、Caspase-3蛋白表达。上述作用均呈浓度依赖。结论SRF可显著抑制HepG2荷瘤裸鼠肿瘤生长并促进肿瘤细胞凋亡,且作用呈浓度依赖。其机制可能通过调控Bax/Bcl-2-Caspase-3通路与抑制信号通路上游NF-κB-p65及VEGF等因子的蛋白质表达有关。Objective To study the effect and mechanism of Shaoyao Ruangan Formula(SRF)regulating Bax/Bcl-2-Caspase-3 pathway to inhibit the growth of liver cancer.Methods HepG2 tumor-bearing nude mouse models were established and randomly divided into the blank control group,the high,medium and low concentration SRF group.After three weeks of gastric perfusion,the tumors were weighed,the apoptosis of tumor cells was observed through the TUNEL method,and the protein expressions of NF-κB-p65,VEGF,Bcl-2,Bax and Caspase-3 in the tissues of different groups were detected by Western-blot method.Results(1)All SRF at variant concentrations can inhibit the growth of liver cancer in HepG2 tumor-bearing nude mice and induce the apoptosis of tumor cells;(2)SRF can decrease the protein expressions of NF-κB-p65,VEGF and Bcl-2 in liver cancer tissues of nude mice,and up-regulate the protein expressions of Bax and Caspase-3.The above-mentioned effects were all in a concentration-dependent manner.Conclusion SRF can significantly inhibit the growth of tumors in HepG2 tumor-bearing nude mice and promote the apoptosis of tumor cells in a concentration-dependent manner.The mechanism may be related to the inhibition of protein expressions of NF-κB-p65 and VEGF,etc.in the upstream of signaling pathway by regulating Bax/Bcl-2-Caspase-3 pathway.

关 键 词：芍药软肝方 肝癌 HEPG2 分子机制 

分 类 号：R735.7[医药卫生—肿瘤]