小麦穗顶部和基部小穗结实粒数的全基因组关联分析  

Genome-Wide Association Analysis of the Grain Number at the Top and Base of Wheat Ears

作　　者：马婕龙 张政 史雨刚[1] 曹亚萍 孙黛珍[1] 王曙光[1] MA Jielong;ZHANG Zheng;SHI Yugang;CAO Yaping;SUN Daizhen;WANG Shuguang(College of Agronomy,Shanxi Agricultural University,Taigu 030801,China;Institute of Wheat,Shanxi Agricultural University,Linfen 041000,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学小麦研究所,山西临汾041000

出　　处：《山西农业科学》2021年第4期425-429,共5页Journal of Shanxi Agricultural Sciences

基　　金：国家自然基金项目(31671607);山西省农业科学院农业科技创新研究课题(YGJPY2003)。

摘　　要：为挖掘与小麦穗顶部和基部结实粒数性状相关的标记位点及优异等位变异,利用106对有多态性的SSR标记,与4个环境下的穗顶部和基部小穗结实粒数进行全基因组关联分析。结果表明,共关联到27对SSR标记,28个显著关联位点,表型解释率在5.73%~30.73%,其中,Xgwm55(6D)、Xgwm95(2A)、Xgwm102(2D)和Xgwm285(3B)分别在4、2、2、2个环境下与小麦穗顶部或基部小穗结实粒数相关联,为稳定的一因多效标记位点;发现优异等位变异位Xgwm132-6B_(128)和Xgwm102-2D_(142)可分别增加穗顶部小穗结实粒数0.14、0.20粒,Xgwm5-3A_(168)、Xgwm161-3D_(153)以及Xcfe172-3D_(144)可分别增加基部小穗结实粒数0.17、0.29、0.34粒。研究结果对于利用标记辅助选择、增加小麦穗粒数、进而提高小麦产量具有重要参考价值。To explore the marker loci and excellent allelic variation related to grain number at the top and base of wheat ears,this paper used 106 pairs of polymorphic SSR markers to perform genome-wide association analysis with grain number at the top and base of wheat ears in 4 environments.The result showed that 27 pairs of SSR markers were associated with 28 significant association sites,and the phenotypic variation interpretation rate(R2)was 5.73%-30.73%.Among them,Xgwm55(6D)、Xgwm95(2A)、Xgwm102(2D)and Xgwm285(3B)were associated with grain number at the top or base of wheat ears in 4,2,2,2 environments,respectively,which were stable one-cause multi effect markers.It was found that the excellent allelic variants Xgwm132-6B_(128) and Xgwm102-2D_(142) could increase grain number at the top of wheat ears by 0.14 and 0.20,respectively,Xgwm5-3A168,Xgwm161-3D_(153) and Xcfe172-3D_(144) could increase grain number at the base of wheat ears by 0.17,0.29,0.34,respectively.Thr research result had important reference value for the use of marker-assisted selection to increase the number of grains per spike and increase wheat yield.

关 键 词：小麦 穗顶部和基部结实粒数 SSR标记 全基因组关联分析 

分 类 号：S512.1[农业科学—作物学]