牛ZC3H12C基因在组织和胎盘中的等位基因表达及DNA甲基化状态分析  

Analyses of Allele Expression and DNA Methylation Status of Bovine(Bos taurus) ZC3H12C Gene in Tissues and Placenta

作　　者：赵广武 刘晓倩 杨子怡 谷书凯 杨欣怡 孟世雄 张萃[1] 李世杰[1] ZHAO Guang-Wu;LIU Xiao-Qian;YANG Zi-Yi;GU Shu-Kai;YANG Xin-Yi;MENG Shi-Xiong;ZHANG Cui;LI Shi-Jie(College of Life Science,Hebei Agricultural University,Baoding 071001,China;College of Food Science,Hebei Agricultural University,Baoding 071001,China)

机构地区：[1]河北农业大学生命科学学院,保定071001 [2]河北农业大学食品科学学院,保定071001

出　　处：《农业生物技术学报》2021年第1期58-66,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31372312);河北省自然科学基金(C2020204004)。

摘　　要：基因组印记(genomic imprinting)作为表观遗传现象,是指依赖亲本来源特异性的单等位基因表达。印记基因在哺乳动物胚胎和胎盘发育以及个体生长中具有重要作用。ZC3H12C (zinc finger CCCHtype containing 12C)基因编码具有CCCH-type锌指结构的转录因子,参与调控人类(Homo sapiens)多种免疫性疾病。ZC3H12C首先在人类胎盘中被发现为印记基因,在牛(Bos taurus)中是否发生印记尚不明确。本研究首先分析牛ZC3H12C基因结构,进而基于SNP比较基因组DNA和cDNA扩增产物的方法分析牛ZC3H12C基因在组织和胎盘中的等位基因表达状态,发现ZC3H12C基因在心脏、肝脏、脾脏、肺脏、肾脏、大脑等6个组织均表现为单等位基因表达,在胎盘中表现为双等位基因表达。进一步利用亚硫酸盐测序法分析ZC3H12C基因的甲基化状态,发现在单等位基因表达的肝脏和肾脏中,X2剪接体的第1个内含子上含有一段差异甲基化区,而在双等位基因表达的胎盘中,该区域呈现轻甲基化。上述结果提示,DNA甲基化可能参与调控牛ZC3H12C基因的单等位基因表达。本研究结果可为深入研究牛ZC3H12C基因的功能提供参考依据。Genomic imprinting is an epigenetic phenomenon that causes genes to be monoallelically expressed dependent on the parent origin. In mammalian, imprinted genes play an important role in placental and embryonic development, as well as in the growth of individuals. The zinc finger CCCH-type containing12 C(ZC3 H12 C) gene encodes a transcription factor containing a single CCCH-zinc finger domain and is involved in regulating a variety of human immune diseases. ZC3 H12 C gene was first identified imprinted in the human(Homo sapiens) placenta, but its imprinting status has not been established in cattle(Bos taurus). In this study, the structure of the bovine ZC3 H12 C gene was first analyzed. Then by the method of comparing genomic DNA and cDNA amplification products based on single nucleotide polymorphism(SNP), the allele expression of ZC3 H12 C gene was analyzed in bovine tissue. The results showed that the ZC3 H12 C gene exhibited monoallelic expression in all 6 tested tissues, including heart, liver, spleen, lung, kidney, and brain, but they showed biallelic expression in the bovine placenta. The methylation status of ZC3 H12 C gene were analyzed by sulfite sequencing method. A differentially methylated region was found in intron 1 of ZC3 H12 C X2 spliced transcript, but in the placenta this region showed hypormethylated. These results suggesting that the monoallele expression of the ZC3 H12 C gene in bovine tissues is regulated by DNA methylation modification.These results provide valuable information on the further study of the function of ZC3 H12 C gene.

关 键 词：ZC3H12C(zinc finger CCCH-type containing 12C)基因 基因组印记 DNA甲基化 牛 

分 类 号：S823[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]