低氧预适应对HT22细胞自噬的影响  

Effects of hypoxic preconditioning on autophagy of HT22 cells

作　　者：赵志军[1] 曹菡 刘宇通 吴俊澄 宝婉婷 张敏[2] 齐瑞芳[3] 邵国[3] Zhao Zhijun;Cao Han;Liu Yutong;Wu Juncheng;Bao Wanting;Zhang Min;Qi Ruifang;Shao Guo(Baotou Medical College of Inner Mongolia University of Science and Technology Department of Neurosurgery in the First Afiliated Hospital,Baotou 014010;Baotou Medical College of Inner Mongolia University of Science and Technology,Baotou 014060;Preclinical and Forensic Medicine,Key Laboratory of Hypoxic Translational Medicine,Baotou 014060,China)

机构地区：[1]内蒙古科技大学包头医学院第一附属医院神经外科,包头014010 [2]内蒙古科技大学包头医学院,包头014060 [3]内蒙古科技大学包头医学院基础医学与法医学院,低氧适应转化医学重点实验室,包头014060

出　　处：《神经解剖学杂志》2021年第1期79-83,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81660307,82060337);内蒙古自治区自然科学基金项目(2019MS03093);内蒙古自治区高等学校科学研究项目(NJZY20171);包头医学院花蕾计划(HL2020001,HL2020006,HL2020014,HL2020027)。

摘　　要：目的:研究低氧预适应(HPC)对小鼠海马HT22细胞自噬的影响。方法:将HT22细胞并分为对照组(control)、低氧组(hypoxia)、低氧预适应组(HPC)和低氧预适应与3-甲基腺嘌呤联合处理组(HPC+3-MA)。MTS法检测HT22细胞活性,采用real time RT-PCR检测HT22细胞中自噬及微管相关蛋白1轻链3(LC3)mRNA表达,利用Western Blot检测LC3蛋白的表达及LC3Ⅱ/LC3Ⅰ的比值变化,转染GFP-LC3进一步检测LC3Ⅱ表达。结果:HPC可增加低氧状态下HT22细胞的活性,与HPC组相比,HPC+3-MA组HT22细胞活性降低;HPC可以诱导低氧状态下HT22细胞中LC3的mRNA的表达,并增加LC3Ⅱ/LC3Ⅰ的比值。结论:HPC通过促进低氧状态下HT22细胞自噬提高细胞活性。Objective:The aim of this study was to explore the effect of hypoxic preconditioning(HPC)on autophagy in mouse hippocampal neuronal HT22 cells.Methods:HT22 cells were divided into four groups:control group,hypoxia group,hypoxia preconditioning group(HPC group),and hypoxic preconditioning which treated with 3-Methyladenine group(HPC+3-MA).Cell viability was measured by MTS method.Microtubule-associated protein1 light chain 3(LC3)mRNA level was detected by real time RT-PCR.The protein expression level of LC3 and LC3Ⅱ/LC3Ⅰratio were tested by Western Blot.The LC3Ⅱwas observed in cells which transfected with GFP-LC3.Results:HPC can increase the viability of HT22 cells under hypoxia.Compared with that in the HPC group,the viability of HT22 cells in the HPC+3-MA group was reduced.HPC can induce the expression of LC3 mRNA in HT22 cells under hypoxia and increased LC3Ⅱ/LC3Ⅰratio.Conclusion:HPC can improve cell viability by promoting autophagy in HT22 cells under hypoxia.

关 键 词：低氧预适应 神经保护 自噬 HT22细胞 

分 类 号：R363[医药卫生—病理学]