核糖体蛋白L29在舌鳞状细胞癌中的表达及意义  

Expression and significance of ribosomal protein L29 in tongue squamous cell carcinoma

作　　者：孔宪琛 姜晓东 芦昕 禹雯怡 薛娇 张鹏[2] 赵璐[2] 袁荣涛[2] KONG Xian-chen;JIANG Xiao-dong;LU Xin;YU Wen-yi;XUE Jiao;ZHANG Peng;ZHAO Lu;YUAN Rong-tao(School of Stomatology,Qingdao University,Qingdao 266003;Stomatologyr Center,Qingdao Municipal Hospital,Qingdao 266071;Department of Stomatology,Qingdao Fifth People’s Hospital.Qingdao 266002,Shandong Province,China)

机构地区：[1]青岛大学口腔医学院,山东青岛266003 [2]青岛大学附属青岛市市立医院口腔医学中心,山东青岛266071 [3]青岛市第五人民医院口腔科,山东青岛266002

出　　处：《中国口腔颌面外科杂志》2021年第2期110-115,共6页China Journal of Oral and Maxillofacial Surgery

基　　金：山东省自然科学基金(ZR2016HM34)。

摘　　要：目的:探讨核糖体蛋白L29(ribosomal protein L29,RPL29)在人舌鳞状细胞癌(简称鳞癌)中的表达及意义。方法 :应用免疫组织化学SP法检测52例舌鳞癌患者癌组织和癌旁组织中RPL29蛋白的表达情况。利用RNA干扰技术,将RPL29-siRNA转染到舌鳞癌CAL-27细胞中,RT-PCR法检测RPL29 mRNA的表达,蛋白质印迹法检测RPL29蛋白的表达,Transwell小室检测细胞的侵袭能力。采用SPSS 21.0软件包对数据进行统计学处理。结果:RPL29在舌鳞癌组织中的表达显著高于癌旁组织,RPL29蛋白表达与肿瘤有、无淋巴结转移相关(P<0.05),与患者性别、年龄、吸烟史、饮酒史、病理分级、T分期无显著相关性(P>0.05)。转染RPL29-siRNA后,舌鳞癌CAL-27细胞中RPL29表达水平下调,CAL-27细胞的侵袭能力显著下降。结论:RPL29在舌鳞癌组织中表达增高,其表达水平与患者有、无淋巴结转移之间差异显著。抑制舌鳞癌CAL-27细胞中RPL29的表达,可降低细胞体外侵袭能力。RPL29在舌鳞癌的侵袭和转移中可能发挥重要作用。PURPOSE:To investigate the expression and significance of ribosomal protein L29(RPL29)in human tongue squamous cell carcinoma(TSCC).METHODS:SP method of immunohistochemistry was carried out to detect the expression of RPL29 in cancer tissues and adjacent tissues of 52 TSCC patients.CAL-27 cells were transfected with RPL29-siRNA.RPL29 protein and mRNA levels were detected using Western blot and RT-PCR.Transwell invasion assay was performed to assess the invasion ability of CAL-27 cells.SPSS 21.0 software package was used for statistical analysis.RESULTS:The expression of RPL29 in TSCC was significantly higher than that in paracancerous tissues.RPL29 overexpression was found to be associated with cervical lymph node metastasis(P<0.05),but not with age,gender,smoking history,alcohol drinking history,pathological type and T stage(P>0.05).After transfecting RPL29-siRNA into CAL-27 cells,the ability of invasion was significantly decreased.CONCLUSIONS:The expression of RPL29 in TSCC was significantly increased and its expression level was significantly different from that in patients with or without lymph node metastasis.The invasive ability of CAL-27 cells was significantly inhibited after transfection with RPL29-siRNA.RPL29 may play an important role in the processes of invasion and metastasis of TSCC.

关 键 词：核糖体蛋白L29 舌鳞状细胞癌 RNA干扰 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]