种植体周围炎大鼠模型研究  

Modification of Rat Model with Peri-implantitis

作　　者：李星佳 陈琪欣 袁长永 王鹏来 LI Xingjia;CHEN Qixin;YUAN Changyong;WANG Penglai(School of Stomatology, Xuzhou Medical University, Xuzhou 221004, China;Department of Oral Implantology, Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou 221000, China)

机构地区：[1]徐州医科大学口腔医学院,江苏徐州221004 [2]徐州医科大学附属口腔医院口腔种植科,江苏徐州221000

出　　处：《口腔医学研究》2021年第4期314-318,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81700954);江苏省研究生科研与实践创新计划项目(编号:KYCX19_2245)。

摘　　要：目的:改良大鼠种植体周围炎模型,简化造模步骤,缩短造模时间。方法:拔除16只大鼠上颌第一磨牙,拔牙窝愈合1个月后植入钛种植体。种植体骨结合4周后用丝线结扎诱导炎症并糖水喂养。结扎后2周观察炎症诱导情况。处死大鼠,取上颌骨拍摄X线片用于影像学分析。制作骨组织切片和提取种植体周围牙龈组织进行免疫组化分析。结果:结扎2周后种植体周围炎诱导成功,种植体周围不仅有软组织炎症,还有不同程度骨组织吸收。结论:大鼠种植体周围炎模型改良成功,造模方便,造模周期短。Objective:To simplify the steps and shorten the time of reconstruction of rat peri-implantitis model.Methods:The maxillary first molars of 16 rats were extracted,and titanium implants were implanted one month later.After 4 weeks,silk thread ligation was used to induce inflammation and the rats were fed with sugar water.Two weeks later,the rats were sacrificed,the maxilla was taken X-rays for imaging analysis,bone tissue slices were made,and the gingival tissue around the implant was extracted for immunohistochemical analysis.Results:Two weeks after ligation,peri-implantitis was successfully induced.There was not only soft tissue inflammation around the implant,but also bone tissue resorption at various degrees.Conclusion:The model of rat peri-implantitis has been successfully constructed,with convenience and short modeling cycle.

关 键 词：种植体周围炎 大鼠 钛种植体 骨结合 

分 类 号：R78[医药卫生—口腔医学]