IGF2BP2的异常表达影响口腔鳞状细胞癌的增殖和侵袭  

Aberrant Expression of IGF2BP2 Promotes Proliferation and Invasion of Oral Squamous Cell Carcinoma

作　　者：胡雅英 陈文 张佳莉 HU Yaying;CHEN Wen;ZHANG Jiali(Hospital of Stomatology, Wuhan University, Wuhan 430079, China;The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei_MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School of Stomatology, Wuhan University. Wuhan 430079, China;Center for Genome Analysis, ABLife Inc, Wuhan 430075, China)

机构地区：[1]武汉大学口腔医院口腔病理科,湖北武汉430079 [2]口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室武汉大学口腔医学院,湖北武汉430079 [3]武汉生命之美科技有限公司基因组分析中心,湖北武汉430075

出　　处：《口腔医学研究》2021年第4期354-359,共6页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:8197103262)。

摘　　要：目的:探讨口腔鳞状细胞癌(OSCC)中异常表达的胰岛素样生长因子2 mRNA结合蛋白2(IGF2BP2)对增殖和侵袭的影响。方法:将4对OSCC组织及癌旁组织进行mRNA高通量测序,筛选差异表达RNA结合蛋白;在OSCC细胞系中利用RNA干扰沉默技术降低IGF2BP2的表达;利用CCK-8和Transwell实验检测IGF2BP2对增殖和侵袭的影响。对HSC3细胞系进行改良RIP高通量测序(Improved RIP-seq,iRIP-seq)和mRNA高通量测序分析IGF2BP2的下游靶基因,运用KEGG信号通路富集分析获得IGF2BP2靶标基因的主要富集信号通路。结果:在OSCC癌组织中IGF2BP2为表达丰度和差异上调最为显著的RNA结合蛋白(P=7.11E-06)。下调IGF2BP2抑制OSCC的体内外增殖和体外侵袭。IGF2BP2的潜在靶标基因主要富集到Axon guidance、Focal adhesion、Rap1信号通路、pathways in cancer、MAPK信号通路等。结论:IGF2BP2在OSCC中表达增高并可能通过影响细胞增殖和粘附相关信号通路发挥促进肿瘤的作用。Objective:To explore the effects of aberrant expressed IGF2BP2 on proliferation and invasion of oral squamous cell carcinoma(OSCC).Methods:High-throughput RNA sequencing was performed on four pairs of OSCC tissues and adjacent normal tissues to screen differentially expressed RNA binding proteins.siRNA was used to reduce the expression of IGF2BP2 in OSCC cell lines.The effects of IGF2BP2 on proliferation and invasion were determined by CCK-8 and Transwell Assay.The target genes of IGF2BP2 were analyzed by Improved RIP-seq and mRNA-seq,and the main signal pathways of IGF2BP2 target genes were obtained by KEGG analysis.Results:The abundance and up-regulation of IGF2BP2 was the most significantly in the RNA-binding proteins in OSCC(P<0.01).The decreased IGF2BP2 inhibited the proliferation and invasion of OSCC cell lines.The target genes of IGF2BP2 were mainly enriched in Axon Guidance,Focal expression,Rap1 signaling pathway,Pathways in Cancer,and MAPK signaling pathway.Conclusion:IGF2BP2 is increased in OSCC and may promote tumor progression by affecting cell proliferation and adherence-related signaling pathways.

关 键 词：胰岛素样生长因子2 mRNA结合蛋白2 口腔鳞状细胞癌 增殖 侵袭 

分 类 号：R73[医药卫生—肿瘤]