黄连根腐病土赤壳属病原真菌的鉴定  

Identification of Coptis chinensis Root Rot Disease Pathogenic Fungi Belonging to Ilyonectria spp.

作　　者：伍晓丽[1,2] 陈大霞[1,2] 刘飞[2,3] 王钰[1,2] 李隆云[1,2] WU Xiao-li;CHEN Da-xia;LIU Fe;WANG Yu;LI Long-yun(Institute of Material Medical Planting,Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China;Chongqing Sub-cen-ter of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Science,Chongqing 400065,China;Health Center of Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)

机构地区：[1]重庆市中药研究院种植研究所,重庆400065 [2]中国中医科学院中药资源中心重庆分中心,重庆400065 [3]重庆市中药研究院大健康中心,重庆400065

出　　处：《西南农业学报》2021年第2期299-305,共7页Southwest China Journal of Agricultural Sciences

基　　金：重庆市基本科研业务费项目(cstc2019jxjl-jbky10004);重庆市卫计委重点项目(ZY201801006);重庆市自然基金项目(cstc2019jcyj-msxmX0677);国家中药材产业技术体系(CARS-21);国家重点研发计划项目(2017YFC1702601)。

摘　　要：【目的】鉴定黄连根腐病的病原,为该病害的防治提供参考依据。【方法】用组织分离法得到病根土赤壳属真菌,通过离体回接初步筛选出疑似病原,通过活体回接鉴定出其中的病原菌,对病原菌的菌落形态、产孢结构、孢子类型和形态等进行观察和测量,同时对其ITS序列测序,在GeneBank中进行同源性比对。【结果】共分离到土赤壳属真菌38株。不同株系的菌株侵染力有较大差异。其中二株菌致病性强,分离频率高,是病原菌。它们的ITS序列与Ilyonectria liliigena和I.destructans的同源性分别达99%,形态特征与这两株菌一致。结合分子和形态鉴定结果,将二者分别鉴定为I.liliigena和I.destructans。此外,部分土赤壳菌的菌落形态和致病性在培养过程中发生了变异,其ITS序列碱基也发生了突变。【结论】I.liliigena和I.destructans是黄连根腐病的病原真菌,这类土赤壳属真菌基因易发生突变。【Objective】The present paper aimed to identify the pathogen of Coptis chinensis root rot disease and supply data for treatment of the disease.【Method】Fungi belonging to Ilyonectria spp.were isolated,inoculated to root and plant,and identified with morphology(colony morphology,sporogenous structure,spore type and morphology et al.)and molecular method(sequencing and alignment of ITS sequence).【Result】38 Ilyonectria strains were isolated from rot root.There was obvious different pathogenicity among the strains.2 strains were dominant and displayed relatively high pathogenicity.The ITS sequences of them showed 99%similarity with I.liliigena and I.destructans and the morphological characteristics of the pathogen were consistent with them.Based on the morphological and molecular characteristics,the pathogen were identified as I.liliigena and I.destructans.Mycelial morphology,pathogenicity and ITS gene of some of the Ilyonectria spp.strains varied during culture.【Conclusion】I.liliigena and I.destructans were the pathogen of C.chinensis root rot disease.Gene mutation occurred frequently in Ilyonectria spp.fungi.

关 键 词：黄连根腐病 病原真菌 土赤壳属 基因突变 

分 类 号：S888.716[农业科学—特种经济动物饲养]