新疆地方绵羊品种Cytb基因遗传多样性与系统进化分析  

Analysis of Genetic Diversity and Phylogenetic Relationship of Xinjiang Indigenous Sheep Based on Cytb gene

作　　者：吕雪峰 许艳丽 郑文新 胡昕 赛迪古丽·赛买提 王乐 LYU Xue feng;XU Yan-li;ZHENG Wen-xin;HU Xin;Saidiguli·Saimeiti;WANG Le(College of Life Science and Technology,Xinjiang University,Xinjiang Urmuqi 830000,China;Institute of Animal Husbandry Quality Standards,Xinjiang Academy of Animal Sciences,Xinjiang Urmuqi 830046,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830000 [2]新疆畜牧科学院畜牧业质量标准研究所,新疆乌鲁木齐830046

出　　处：《西南农业学报》2021年第2期421-429,共9页Southwest China Journal of Agricultural Sciences

基　　金：国家绒毛用羊产业技术体系(CARS-39-20)。

摘　　要：【目的】为全面了解新疆地方绵羊品种的遗传多样性和系统发育关系,为后期选种、保种和种质资源合理利用提供理论依据。【方法】对新疆13个地方绵羊品种线粒体DNA细胞色素b(cytochrome b,cytb)基因序列进行PCR扩增和测序分析。【结果】除和田羊外,其余12个品种遗传多样性均较丰富;中性检验显示和田羊经历过种群扩张;遗传距离和聚类分析显示和田羊与策勒黑羊遗传距离最近,与巴尔楚克羊遗传距离最远,其它品种与巴尔楚克羊的遗传距离均较远;单倍型网络图分析显示,13个地方品种分为3个分支;分子方差分析表明,群体内变异占91.33%,群体间变异占8.67%,品种间分化不明显。【结论】新疆13个地方绵羊品种有3个母系起源,其亲缘关系与育成史和地理分布基本一致,和田羊的遗传多样性较低,需要加强遗传资源保护。【Objective】The present paper aimed to fully understand the genetic diversity and phylogenetics of Xinjiang indigenous sheep breeds,and provide theoretical basis for breeds,conservation and utilization.【Method】The squences of mitochondrial DNA(mtDNA)Cytb of 13 Xinjiang indigenous sheep breeds were amplified and sequenced.【Result】It was found that these breeds had abundant genetic diversity,except Hotan sheep.Neutral test showed that Hotan sheep had experienced population expansion.The genetic distance between Hotan sheep breeds and Qira black sheep breeds was the closest,and between Hotan sheep breeds and Bachu sheep breeds was the furthest.There were relatively far genetic distance between Bachu sheep breeds and other breeds.13 indigenous sheep breeds were divided into 3 branches by haplotype network.After AMOVA molecular variation analysis,genetic variation between populations and within populations was 91.33%and 8.67%,respectively.There was no significant genetic differentiation among populations.【Conclusion】The results showed that there were three matrilineal origins in 13 Xinjiang indigenous sheep breeds.The genetic relationship was basically consistent with the breeding history and geographical distribution.Hotan sheep had low genetic diversity and need to strengthen the protection of germplasm resources.

关 键 词：绵羊 CYTB 遗传多样性 系统进化 

分 类 号：S826[农业科学—畜牧学]