小细胞肺癌患者外周血辅助性T细胞的表达情况及临床意义  

Expression and clinical significance of helper T cells in peripheral blood of patients with small cell lung cancer

作　　者：岳龙涛[1] 郑盈盈[2] 孙青[1] YUE Longtao;ZHENG Yingying;SUN Qing(Pathology Department,the First Affiliated Hospital of Shandong First Medical University/Shandong Provincial Qianfoshan Hospital,Jinan 250014,China;Medical Research Center,the First Affiliated Hospital of Shandong First Medical University/Shandong Provincial Qianfoshan Hospital,Jinan 250014,China)

机构地区：[1]山东第一医科大学第一附属医院/山东省千佛山医院病理科,山东济南250014 [2]山东第一医科大学第一附属医院/山东省千佛山医院医学研究中心,山东济南250014

出　　处：《临床医学研究与实践》2021年第10期14-17,共4页Clinical Research and Practice

基　　金：山东省重大科技创新工程项目(No.2017CXGC1201);山东省医药卫生科技发展计划项目(No.2015WS0233)。

摘　　要：目的研究小细胞肺癌(SCLC)患者外周血中辅助性T细胞1(Th1)、Th2、Th17及调节性T淋巴细胞(Treg)的表达情况,探讨其在SCLC进展中的作用。方法选择2016年1月至2018年4月本院收治的44例住院的SCLC患者(SCLC组)及24名健康者(对照组)作为研究对象。采用流式细胞法检测两组研究对象外周血中Th1、Th2、Th17及Treg细胞的表达情况,应用微量样本多指标流式蛋白定量技术(CBA)检测血清中的细胞因子IFN-γ、IL-17及IL-10的表达情况。结果SCLC组患者的外周血中Th1细胞、Th1/Th2、IFN-γ表达水平显著低于对照组,差异具有统计学意义(P<0.01)。SCLC组患者的外周血中Th2、Treg、Th17、Treg/Th17、IL-17、IL-10表达水平显著高于对照组,差异具有统计学意义(P<0.01)。结论SCLC患者外周血中Th1/Th2的降低、Treg/Th17的升高可能与SCLC的致病机理及预后密切相关,该结论将为SCLC的治疗提供新的思路。Objective To study the expression of T helper 1 cell(Th1),Th2,Th17 and regulatory T cell(Treg)in peripheral blood of patients with small cell lung cancer(SCLC),and to explore their role in the progression of SCLC.Methods From January 2016 to April 2018,44 inpatients with SCLC(SCLC group)admitted in the hospital and 24 healthy people(control group)were selected as the research objects.Flow cytometry was used to detect the expression of Th1,Th2,Th17 and Treg cells in peripheral blood of the research objects of the two groups,and cytometric bead arrays(CBA)was used to detect the expression of cytokines IFN-γ,IL-17 and IL-10 in serum.Results The expression levels of Th1 cells,Th1/Th2 and IFN-γin peripheral blood of the SCLC group were significantly lower than those of the control group,and the differences were statistically significant(P<0.01).The expression levels of Th2,Treg,Th17,Treg/Th17,IL-17 and IL-10 in peripheral blood of the SCLC group were significantly higher than those of the control group,and the differences were statistically significant(P<0.01).Conclusion The decrease of Th1/Th2 and the increase of Treg/Th17 in peripheral blood of SCLC patients may be closely related to the pathogenesis and prognosis of SCLC,this conclusion will provide a new idea for the treatment of SCLC.

关 键 词：小细胞肺癌 外周血 TH1 TH2 TH17 TREG 

分 类 号：R734.2[医药卫生—肿瘤]