过表达血管生成抑制蛋白1对人结直肠癌细胞恶性生物学行为的影响  

Effect of over-expression of vasohibin-1 on malignant biological behavior of human colorectal cancer cells

作　　者：高卫峰 张春泽[1,2] 张庆怀 孙静[3] 苏延军[4] 张伟华[1] GAO Weifeng;ZHANG Chunze;ZHANG Qinghuai;SUN Jing;SU Yanjun;ZHANG Weihua(Department of Colorectal Surgery,Tianjin Union Medical Center,Tianjin 300121,China;State Key Laboratory of Medicinal Chemical Biology,Nankai University,Tianjin 300071,China;Department of Laboratory,Tianjin Union Medical Center,Tianjin 300121,China;Department of Lung Cancer,Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,China)

机构地区：[1]天津市人民医院肛肠外科,天津300121 [2]南开大学药物化学生物学国家重点实验室,天津300071 [3]天津市人民医院检验科,天津300121 [4]天津医科大学肿瘤医院肺部肿瘤科,天津300060

出　　处：《中国肿瘤生物治疗杂志》2021年第2期121-127,共7页Chinese Journal of Cancer Biotherapy

基　　金：天津市卫生健康委员会项目资助(No.2014KZ053,No.2017057);天津市科技支撑重点研发计划项目资助(No.19YFZCSY00420);南开大学国家重点实验室开放课题资助项目(No.2018094);天津市重大疾病防治科技重大专项资助项目(No.18ZXDBSY00040);天津市人民医院院级课题资助项目(No.2016YJ035)。

摘　　要：目的:探讨过表达血管生成抑制蛋白1(vasohibin-1,VASH1)对人结直肠癌细胞恶性生物学行为的影响。方法:包装慢病毒并感染人结直肠癌SW680、SW620细胞以构建过表达VASH1的细胞系,以未经感染的细胞为对照;qPCR实验和WB实验检测VASH1的过表达效果,小管形成实验、CCK-8实验、软琼脂克隆形成实验、Transwell实验和划痕愈合实验检测过表达VASH1在体外对细胞的微血管形成、增殖、克隆形成以及迁移能力的影响,NOD-SCID小鼠皮下成瘤实验检测过表达VASH1对SW620细胞移植瘤的体内生长和肺转移的影响。结果:成功构建过表达VASH1的SW480和SW620细胞。体外实验表明,与对照组相比,过表达VASH1的结直肠癌细胞的微血管形成能力、增殖能力、克隆形成能力以及迁移能力均显著降低(均P<0.05)。体内实验表明,与对照组相比,过表达VASH1的SW620细胞NOD-SCID小鼠皮下移植瘤的生长速度以及肺转移能力也明显降低(均P<0.05)。结论:过表达VASH1能够抑制人结直肠癌细胞的恶性生物学行为。Objective:To investigate the effects of over-expressing VASH1 on the malignant biological behaviors of human colorectal cancer cells.Methods:Lentivirus was packaged and transfected into human colorectal cancer cells SW680 and SW620 to construct an over-expressed VASH1 cell line,the untransfected cells were used as control.qPCR experiment and WB experiment were used to detect the over-expression effect of VASH1.The effects of VASH1 over-expressed on microangiogenesis,proliferation,colony formation and migration of colorectal cancer cells were detected respectively by tubule formation,CCK-8 assay,soft agar assay,Transwell assay and Wound healing assay in vitro.In addition,tumor growth and lung metastasis were detected in NOD-SCID mice subcutaneously injected with VASH1-overexpressing SW620 cells.Results:Successfully constructed SW480 and SW620 cells over-expressing VASH1.Compared with the control group,the abilities of microangiogenesis,proliferation,colony forming and migration were significantly reduced in colorectal cancer cells over-expressing VASH1(P<0.05)in vitro.The abilities of subcutaneous tumor growth and lung metastasis of colorectal cancer cells over-expressing VASH1 were also significantly reduced(P<0.05)in vivo.Conclusion:Over-expression of VASH1 can suppress the malignant biological behaviors of human colorectal cancer cells.

关 键 词：结直肠癌 血管生成抑制因子1 微血管形成 增殖 转移 

分 类 号：R735.3[医药卫生—肿瘤] R730.54[医药卫生—临床医学]