银杏扩展蛋白基因的鉴定与特征分析  

Identification and Characterization of the Expansin Gene in Ginkgo biloba

作　　者：王瑞雪 杨锐霞 尹鹏 刘家福 徐吉臣[1] Wang Ruixue;Yang Ruixia;Yin Peng;Liu Jiafu;Xu Jichen(National Engineering Laboratory for Tree Breeding,Beijing Forestry University,Beijing,100083)

机构地区：[1]北京林业大学林木育种国家工程实验室,北京100083

出　　处：《分子植物育种》2021年第6期1741-1749,共9页Molecular Plant Breeding

基　　金：中央高校基本科研业务费专项资金(2015ZCQ-SW-01)资助。

摘　　要：扩展蛋白是植物细胞壁重要的组成部分,在植物生长发育和逆境抗性等方面发挥着重要的作用。为探讨银杏(Ginkgo biloba)中扩展蛋白基因家族的组成和特征,本研究利用扩展蛋白的保守结构域,从银杏基因组中鉴定了28个扩展蛋白基因,包括20个EXPA基因、1个EXPB基因、4个EXLA基因和3个EXLB基因。银杏扩展蛋白基因的编码区长度在684~837 bp之间,编码227~278个氨基酸的蛋白质,具有保守的功能域、基序和氨基酸。不同亚家族基因在序列一致性、保守基序及内含子-外显子结构组成上有所不同,呈现亚家族特征。密码子分析显示,扩展蛋白基因具有密码子使用偏性,包含8个高频密码子。银杏扩展蛋白基因信息将有助于理解其在银杏基因组中的地位和作用,为深入研究功能提供基础。Expansins are important components in the plant cell wall,and play an indispensable role in plant growth,development,biotic and abiotic stress resistance.For understanding the composition and characteristics of the expansin gene family in Ginkgo biloba,the study here identified 28 expansin genes in Ginkgo biloba genome base on their conserved structural domains,including 20 EXPA genes,1 EXPB gene,4 EXLA genes and 3 EXLB genes.The gene ORF was in 684~837 bp coding for 227~278 aa with several conserved domains,motifs,and amino acids.Meanwhile,some differences were also present among the genes in subfamilies in sequence identity,motif,and intron-exon structure.A total of 8 high frequency condons were identified in Ginkgo expansin genes,indicating the presence of the codon usage bias.All the information here would help us to understand well the situation and role of expansin genes in Ginkgo biloba genome,and further gain more insight in the future study of the expansin gene function.

关 键 词：银杏(Ginkgo biloba) 扩展蛋白 亚家族 密码子 

分 类 号：S792.95[农业科学—林木遗传育种]