篦子三尖杉内生真菌的分离鉴定和抑菌活性分析  

Isolation,Identification and Antimicrobial Activity Analysis of Endophytic Fungi from Cephalotaxus oliveri

作　　者：韩洁 桂艳玲 付强 邹颉 赵杰宏 HAN Jie;GUI Yanling;FU Qiang;ZOU Jie;ZHAO Jiehong(College of Basic medicine/College of pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550002,China)

机构地区：[1]贵州中医药大学基础医学院/药学院,贵州贵阳550025

出　　处：《山地农业生物学报》2021年第1期66-69,共4页Journal of Mountain Agriculture and Biology

基　　金：三尖杉药用内生真菌诱变选育及其药理活性研究(黔科合J字[2012]2065号);篦子三尖杉内生真菌发酵产物的活性研究(黔科合中药字[2011]LKZ7028号)。

摘　　要：珍稀濒危药用植物篦子三尖杉(Cephalotaxus oliveri Mast)是临床白血病药物三尖杉酯碱和高三尖杉酯碱的重要来源,因此,篦子三尖杉的内生真菌具有潜在的药用价值。通过从篦子三尖杉中分离纯化,筛选出16株外观差异较大的菌株,其中4株无产孢结构。经显微结构和ITS分子鉴定,发现其中11株属于链格孢属。抑菌试验表明,10号菌株对金黄色葡萄球菌的抑制作用较大,11号对白葡萄球菌的抑制作用较大;11号和12号对枯草芽孢杆菌的抑制作用较大;7号对大肠杆菌的抑制作用较大;有5株真菌的抑菌谱较广。筛选的内生真菌具有药用活性,为进一步筛选有药用价值的内生真菌及其代谢产物奠定基础。The rare and endangered medicinal plant Cephalotaxus oliveri Mast is an important source of Harringtonine and Homoharringtonine for clinical leukemic therapy,so that the endophytic fungi of C.oliveri have somewhat potential medicinal value.Here,there were sixteen strains with different appearance screened out by isolation and purification from the leaves and branches of C.oliveri.Among them,there were four strains without sporulation structure,and eleven strains identified as Alternaria by microscopic morphology and ITS identification.The results of bacteriostatic experiment showed that the No.10 strain had greater inhibitory effects on Staphylococcus aureus.The same inhibitory effects were also found for No.11 strain on Staphylococcus albus,the No.11 stain and the No.12 strain on Bacillus subtilis,and the No.7 strain on Escherichia coli.Among which,there were five strains with a wide spectrum of bacteriostatic activity.It provides basis for further screening endophytic fungi for medicinal use.

关 键 词：篦子三尖杉 内生真菌 ITS 抑菌活性 

分 类 号：R931.2[医药卫生—生药学]