扶正消瘤颗粒对人表皮生长因子受体2阳性乳腺癌细胞迁移的实验研究  

Experimental Study of FuzhengXiaoliu Granule on Cell Migration of Her–2 Positive Breast Cancer SKBR–3 Cell Line

作　　者：田欢[1] 岳双冰[1] 蔡俊媛 陈启庭[1] 张子理[1] TIAN Huan;YUE Shuang-bing;CAI Jun-yuan;CHEN Qi-ting;ZHANG Zi-li(Shenzhen Second People’s Hospital,the First Affiliated Hospital of Shenzhen University,Guangdong Shenzhen 518035)

机构地区：[1]深圳市第二人民医院,深圳大学第一附属医院,广东深圳518035

出　　处：《深圳中西医结合杂志》2021年第2期4-7,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：深圳市科技创新委员会科技研发资金资助课题(JCYJ20140414170821153)。

摘　　要：目的:观察扶正消瘤颗粒对人表皮生长因子受体2(Her–2)阳性乳腺癌细胞SKBR–3迁移的影响及其作用机理。方法:SKBR–3细胞以2×10^(6)·2 mL^(-1)的密度接种到6孔培养板中,共5组。72 h后损伤细胞,并加入1倍、2倍、3倍剂量的扶正消瘤颗粒含药血清,以曲妥珠单抗为阳性对照,空白血清为空白对照。加药后24 h、48 h和72 h细胞划痕实验观察扶正消瘤颗粒对SKBR–3细胞迁移的影响;SKBR–3细胞培养48 h,加入上述药物,24 h后Western blot检测Her–2蛋白的表达。结果:扶正消瘤颗粒和曲妥珠单抗抑制SKBR–3细胞迁移的作用明显,24 h、48 h和72 h三个时间点,与空白对照组比较,差异具有统计学意义(P<0.05~0.01),Western blot结果显示扶正消瘤颗粒1倍剂量组和2倍剂量组对SKBR–3细胞Her–2蛋白表达无明显作用,与空白对照组比较,差异均无统计学意义(P>0.05);扶正消瘤颗粒3倍剂量组抑制Her–2蛋白的表达,与空白对照组比较,差异具有统计学意义(P<0.05)。曲妥珠单抗明显抑制Her–2蛋白表达,与扶正消瘤颗粒1倍剂量组和2倍剂量组及空白对照组比较,差异均具有统计学意义(P<0.01)。结论:扶正消瘤颗粒能明显抑制乳腺癌SKBR–3细胞迁移,3倍剂量的扶正消瘤颗粒和曲妥珠单抗对SKBR–3细胞Her–2蛋白表达具有抑制作用。Objective To observe the effect and mechanism of Fuzheng Xiaoliu Granule on the migration of Her-2 positive breast cancer SKBR–3 cell line.Methods 5 groups of SKBR-3 cells were plated at the density of 2×10^(6)·2 mL^(-1)into 6 well culture plates and injured at 72 h,1 times dosage,2 times dosage and 3 times dosage of Fuzhengxiaoliu granule containing serum were added in,Trastuzumab and blank serum were used as positive and blank control.After dosing 24 h、48 h、72 h,Cell Scratch test were used to detect the cell migration of SKBR-3 cells;SKBR–3 cells were cultured for 48 hours,and the expression of Her–2 protein was detected by Western blot after 24 hours of treatment.Results The inhibitory effects of Fuzheng Xiaoliu Granule and Trastuzumab on the migration of SKBR-3 cells were signifi cant,Compared with the Blank control group,the difference were statistically signifi cant in 24 h,48 h and 72 h(P<0.05~0.01);Western blot showed that 1 times dosage and 2 times dosage of Fuzheng Xiaoliu granule had no effect on the expression of Her-2 protein in SKBR-3 cells,compared with blank control group,there were no signifi cant difference(P>0.05);The expression of Her-2 protein in the 3 times dose group of Fuzheng Xiaoliu Granule was signifi cantly lower than that in the blank control group(P<0.05);Trastuzumab signifi cantly inhibited Her-2 protein expression,Compared with 1 times dosage,2 times dosage of Fuzheng Xiaoliu granule and the Blank control group,the difference were statistically signifi cant(P<0.01).Conclusion FuZheng XiaoLiu granule obviously inhibited the migration of breast cancer SKBR-3 cells;3 times dose of Fuzheng Xiaoliu Granule and Trastuzumab inhibited the expression of Her-2 protein in SKBR-3 cells.

关 键 词：乳腺癌 扶正消瘤颗粒 SKBR–3细胞株 人表皮生长因子受体2 

分 类 号：R737.9[医药卫生—肿瘤]