膨压处理对冬枣薄壁组织细胞微观力学特性的影响  

Effects of Turgor Treatments on Micromechanical Properties of Parenchyma Cells of Winter Jujube

作　　者：朱韵昇 张海红[1] 邱雪 吴宝婷 Zhu Yunsheng;Zhang Haihong;Qiu Xue;Wu Baoting(College of Agriculture,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《中国食品学报》2021年第3期237-244,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31860422)。

摘　　要：为研究不同膨压处理对冬枣薄壁组织细胞微观力学特性的影响,分别使用0.1,0.45,0.8 mol/L的甘露醇溶液对冬枣薄壁组织进行渗透处理,使冬枣薄壁细胞处于不同的膨压状态。利用原子力显微镜对不同膨压下的冬枣薄壁细胞进行纳米压痕试验,分析比较不同膨压下冬枣薄壁细胞的微观力学特性。结果表明:高膨压、常膨压、低膨压下的杨氏模量E值分别是:(6.76±7.57),(20.21±16.95)kPa和(5.58±4.24)kPa,常膨压杨氏模量E值与低、高膨压的杨氏模量E值相比有显著性差异(P<0.05)。表观刚度值从高膨压到低膨压分别是:(0.013±0.003),(0.018±0.004),(0.008±0.002)N/m,其变化趋势与杨氏模量一致,表明杨氏模量E值可反映表观刚度的大小。高膨压、常膨压、低膨压下的黏附力分别是(102.06±163.55),(178.41±141.59),(187.96±76.17)pN,且高膨压时黏附力与常、低膨压的黏附力相比有显著性差异(P<0.05)。不同膨压会导致冬枣薄壁组织细胞微观力学特性差异,本研究结果为冬枣采后贮藏保鲜提供了理论依据。To study the effect of different turgor treatment on micromechanics of parenchyma cells of Winter jujube.In this paper,mannitol solutions of 0.1,0.45,0.8 mol/L were used to treat the parenchyma of Winter jujube.The parenchyma cells of Winter jujube were in different turgor states.AFM was used to conduct nano indentation test on parenchyma cells of Winter jujube at different turgor.The effects of different turgor pressure on the young’s modulus,apparent stiffness and adhesion of tissue cells in Winter jujube were analyzed.The results showed that:The young’s modulus E under high turgor pressure and normal turgor pressure and low turgor pressure were respectively:(6.76±7.57)kPa,(20.21±16.95)kPa,(5.58±4.24)kPa.The young’s modulus E value of normal turgor had a significant difference from that of low and high turgor(P<0.05).The apparent stiffness value k ranges from high turgor to low turgor:(0.013±0.003)N/m,(0.018±0.004)N/m,(0.008±0.002)N/m,and its variation trend was consistent with young’s modulus,indicating that young’s modulus E can reflect the apparent stiffness.Adhesion forces at high turgor,normal turgor and low turgor were(102.06±163.55)pN,(178.41±141.59)pN,(187.96±76.17)pN,respectively.The adhesion at high turgor pressure was significantly different from that at normal and low turgor pressure(P<0.05).Different turgor pressure may lead to different micromechanical properties of parenchyma cells of Winter jujube.

关 键 词：冬枣薄壁细胞 膨压 微观力学 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]