高级别浆液性卵巢癌中膜突蛋白的表达与意义  

Expression and clinical relevance of moesin in high-grade serous ovarian cancer

作　　者：祝彩霞[1] 杨娟[1] 袁林静 牛刚[1] Zhu Caixia;Yang Juan;Yuan Linjing;Niu Gang(Department of Obstetrics and Gynecology,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学附属第一医院妇产科,广州510080

出　　处：《新医学》2021年第4期265-271,共7页Journal of New Medicine

基　　金：国家自然科学基金青年科学基金(81702550)。

摘　　要：目的检测膜突蛋白(MSN)在进展型高级别浆液性卵巢癌(HGSOC)中的表达情况,分析MSN表达上调对卵巢腺癌细胞增殖和侵袭能力的影响。方法在124例进展型HGSOC标本中应用逆转录PCR检测MSN mRNA的表达情况,分析其与临床特征和预后的关系。体外培养卵巢腺癌细胞OVMZ-6,转染过表达MSN后应用逆转录PCR和蛋白免疫印迹法验证MSN表达变化;分别采用四甲基偶氮唑蓝(MTT)法、Transwell小室实验和流式细胞仪检测MSN表达上调后对卵巢腺癌细胞增殖和侵袭能力的影响。结果进展型HGSOC标本均能检出MSN mRNA的表达,Kaplan-Meier生存分析显示MSN mRNA高表达者总生存期高于MSN mRNA低表达者(P<0.05),但无进展生存期组间比较差异无统计学意义(P>0.05)。多因素Cox回归分析显示MSN mRNA高表达是总生存期的独立保护因素(HR=0.51,95%CI 0.31~0.84,P=0.009)。MSN-pRc/RSV转染后,OVMZ-6细胞中MSN mRNA和蛋白表达水平显著上调。MSN过表达的OVMZ-6细胞增殖能力和侵袭能力明显下降(P均<0.05)。结论进展型HGSOC中MSN表达上调可抑制卵巢腺癌细胞的增殖和侵袭能力,影响患者总生存期,提示MSN可能是进展型HGSOC精准治疗的新靶点。Objective To detect the expression level of moesin(MSN)and clinical relevance in advanced high-grade serous ovarian cancer(HGSOC),and to evaluate the effect of MSN overexpression on cell proliferation and invasiveness in ovarian cancer cell line OVMZ-6.Methods The expression level of MSN mRNA in 124 advanced HGSOC specimens was detected by reverse transcription-polymerase chain reaction(RT-PCR),and its correlation with clinical characteristics and prognosis was analyzed.After stable transfection with overexpressed MSN,the expression level of MSN mRNA and protein of ovarian cancer cell line OVMZ-6 were determined by RT-PCR and Western blot.The effect of MSN on cell proliferation and invasiveness was evaluated by MTT assay,Transwell chamber assay and flow cytometry in vitro.Results MSN mRNA could be detected in all HGSOC tissues.Kaplan-Meier survival analysis showed that elevated MSN mRNA expression predicted longer overall survival(OS)(P<0.05),whereas there was no significant association between MSN mRNA and progression-free survival(PFS)(P>0.05).Multivariate Cox’s regression analysis demonstrated increased MSN mRNA expression was identified as an independent favorable prognostic factor for OS(HR=0.51,95%CI 0.31~0.84,P=0.009).The expression levels of MSN mRNA and protein were significantly up-regulated after transfection with MSN-pRc/RSV.The cell proliferation and invasiveness were significantly inhibited in ovarian cancer cells transfected with MSN overexpression(all P<0.05).Conclusions Overexpression of MSN can suppress cell proliferation and invasiveness of ovarian cancer cells,resulting in longer OS in patients diagnosed with advanced HGSOC.MSN might serve as a novel therapeutic target in advanced HGSOC.

关 键 词：高级别浆液性卵巢癌 膜突蛋白 细胞增殖 侵袭 预后 

分 类 号：R737.31[医药卫生—肿瘤]