长链非编码RNA MIAT在肝细胞癌组织中的表达及临床意义  

Expression and significance of long non-coding RNA MIAT in hepatocellular carcinoma

作　　者：赵金磊[1] 季春勇[1] 石伟[1] 罗红杰 季予江[1] 刘东亮[1] ZHAO Jinlei;JI Chunyong;SHI Wei;LUO Hongjie;JI Yujiang;LIU Dongliang.(Department of Minimally Invasive Hepatobiliary and Pancreatic Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 453000, China)

机构地区：[1]郑州大学附属郑州市中心医院肝胆胰微创外科,郑州453000

出　　处：《临床肿瘤学杂志》2021年第3期217-221,共5页Chinese Clinical Oncology

摘　　要：目的探讨长链非编码RNA MIAT在肝细胞癌(HCC)组织中的表达水平及与临床病理特征和预后的关系。方法收集2012年5月至2015年5月接受手术治疗的HCC患者103例,采用实时荧光定量PCR(qPCR)检测HCC和配对癌旁组织中MIAT的相对表达量。以HCC组织中MIAT表达量的P25值为界值将患者分为低表达组和高表达组,并进行生存分析。影响HCC患者总生存时间(OS)的多因素分析采用Cox比例风险回归模型。结果HCC组织中MIAT的相对表达量为2.38±0.16,高于癌旁组织的1.02±0.11,差异有统计学意义(P<0.001)。MIAT表达水平与性别、年龄、乙肝表面抗原、肝硬化、肿瘤数量、肿瘤大小、肿瘤部位和甲胎蛋白(AFP)水平无关(P>0.05),与TNM分期、淋巴结转移、门静脉癌栓和分化程度有关(P<0.05)。全组中位OS为25.0个月。低表达组(n=27)的中位OS和5年生存率分别为未达和55.6%,均高于高表达组(n=76)的21.0个月和15.8%,差异有统计学意义(P<0.001)。Cox多因素分析显示,TNM分期、淋巴结转移和MIAT表达是影响HCC患者OS的独立因素(P<0.05)。结论MIAT在HCC组织中表达升高,且与肿瘤的进展和转移有关,是影响患者OS的独立因素。Objective To investigate the expression of long non-coding RNA MIAT in hepatocellular carcinoma(HCC)tissues,and its relationship with clinicopathological factors and prognosis.Methods A total of 103 cases of patients with HCC who underwent elective surgical treatment from May 2012 to May 2015 were enrolled.The expression of MIAT in HCC and adjacent tissues were detected by quantitative real-time PCR(qPCR).Using the P25 value of MIAT expression in HCC tissues as the cutoff value,patients were divided into the low expression group and high expression group for further survival analysis.Cox regression model was used to analyze the factors affecting the overall survival(OS)of patients with HCC.Results The expression level of MIAT in HCC tissues was 2.38±0.16,higher than 1.02±0.11 in adjacent tissues,and the difference was statistically significant(P<0.001).The expression of MIAT was not related to gender,age,hepatitis B surface antigen,cirrhosis,number of tumors,tumor size,tumor location and alpha fetoprotein level(P>0.05),but with differentiation,TNM stage,lymph node metastasis and portal vein tumor thrombus(P<0.05).The median OS of 103 cases of HCC patients was 25.0 months.The median OS and 5-year survival rate of patients in low expression group(n=27)were unachieved and 55.6%,respectively,which were higher than 21.0 months and 15.8%in high expression group(n=76),and the differences were significant(P<0.001).The results of multivariate Cox regression model analysis further showed that TNM stage,lymph node metastasis and the expression of MIAT were independent factors affecting OS of patients with HCC(P<0.05).Conclusion The expression of MIAT in HCC tissues is elevated,and is related to tumor progression and metastasis.It is an independent factor affecting the OS of patients.

关 键 词：肝细胞癌 长链非编码RNA MIAT 预后 

分 类 号：R735.7[医药卫生—肿瘤]