Developmental and Liver Metabolite Changes Induced by TPhP Exposure in Brown Frog(Rana zhenhaiensis)Tadpoles  

作　　者：Hongliang LU Yingchao HU Changqing XU Wei DANG Zhihua LIN 

机构地区：[1]Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration,School of Life and Environmental Sciences,Hangzhou Nomal University,Hangzhou 311121.Zhejiang,China [2]Medical School,Hangzhou Normal University,Hangzhou 311121,Zhejiang,China [3]College of Ecology,Lishui University,Lishui 323000,Zhejiang,China

出　　处：《Asian Herpetological Research》2021年第1期135-142,共8页亚洲两栖爬行动物研究（英文版）

基　　金：a grant from the Natural Science Foundation of Zhejiang Province(LY15C030006)。

摘　　要：As an organophosphorus compound that frequently detected in water samples,triphenyl phosphate(TPhP)has been showed to have multiple toxicological effects on aquatic species.However,no attention has been paid to its potential impact on non-model amphibian species.Here,tadpoles of the Zhenhai brown frog(Rana zhenhaiensis)were exposed to different concentrations of TPh P(0,0.02 and 0.1 mg/L)throughout the developmental period to assess physiological and meta bolic impacts of TPh P exposure on amphibian larvae.After 30-day TPh P exposure,the developmental stage of tadpoles from the high-concentration treatment appeared to be more advanced than that from the other two treatments,but other measured traits(including body size,tail length and liver weight)did not differ among treatments.Metabolite profiles in tadpole livers based on liquid chromatographymass spectrometry(LC-MS)revealed a distinct metabolic disorder in exposed animals.Specifically,significant changes in various hepatic amino acids(such as glutamine,glutamate,valine and leucine)were observed.Overall,our results indicated that chronic TPhP exposure potentially caused developmental and hepatic physiological changes in R.zhenhaiensis tadpoles,although its impact on tadpole growth appeared to be minor.

关 键 词：larval development liver metabolite triphenyl phosphate exposure Zhenhai brown frog 

分 类 号：X171.5[环境科学与工程—环境科学]